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验证数据展示

  • IF Staining of A431 using CL555-18343

    IF Staining of A431 using CL555-18343

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 555 Cytokeratin 10 antibody (CL555-18343) at dilution of 1:200.

  • FC experiment of A431 using CL555-18343

    FC experiment of A431 using CL555-18343

    1X10^6 A431 cells were intracellularly stained with 0.5 ug CoraLite® Plus 555 Anti-Human Cytokeratin 10 (CL555-18343) (red), or 0.5 ug CL555 Rabbit Isotype Control Antibody (CL555-30000)(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of mouse skin using CL555-18343

    IF Staining of mouse skin using CL555-18343

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using CoraLite® Plus 555 Cytokeratin 10 antibody (CL555-18343) at dilution of 1:50. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite® Plus 555-conjugated Cytokeratin 10 Polyclonal antibody

Cytokeratin 10 Polyclonal Antibody for IF/ICC, IF-P, FC (Intra)
Cat No. CL555-18343

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, IF-P, FC (Intra)

别名

KRT10, K10, Cytokeratin-10, Cytokeratin,Keratin, CK-10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 555
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse skin tissue
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy KRT10 Ready-To-Use IHC Kit. KRT10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 18343-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-18343 targets Cytokeratin 10 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, IF-P, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Cytokeratin 10 fusion protein Ag13136 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 10
别名 KRT10, K10, Cytokeratin-10, Cytokeratin,Keratin, CK-10
计算分子量 584 aa, 59 kDa
GenBank蛋白编号BC034697
基因名称 Cytokeratin 10
Gene ID (NCBI) 3858
RRIDAB_3084531
偶联类型 CoraLite® Plus 555 Fluorescent Dye
最大激发/发射波长554 nm / 570 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13645
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a type I keratin, keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia.

实验方案

Product Specific Protocols
IF protocol for CL Plus 555 Cytokeratin 10 antibody CL555-18343Download protocol
FC protocol for CL Plus 555 Cytokeratin 10 antibody CL555-18343Download protocol
Standard Protocols
Click here to view our Standard Protocols