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CoraLite® Plus 647-conjugated Cytokeratin 10 Polyclonal antibody

Cytokeratin 10 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-18343

Print datasheet

Synonyms

Cell and organelle markers, CK 10, CK10, Cytokeratin 10, Cytoskeleton Marker, K10, keratin 10, KPP, KRT10

验证数据展示

  • FC experiment of A431 using CL647-18343

    FC experiment of A431 using CL647-18343

    1x10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647-conjugated Cytokeratin 10 Polyclonal antibody (CL647-18343)(red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy KRT10 Ready-To-Use IHC Kit. KRT10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 18343-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-18343 targets Cytokeratin 10 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Cytokeratin 10 fusion protein Ag13136 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 10
Synonyms Cell and organelle markers, CK 10, CK10, Cytokeratin 10, Cytoskeleton Marker, K10, keratin 10, KPP, KRT10
Calculated Molecular Weight 584 aa, 59 kDa
GenBank Accession NumberBC034697
Gene Symbol Cytokeratin 10
Gene ID (NCBI) 3858
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13645
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a type I keratin, keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 Cytokeratin 10 antibody CL647-18343Download protocol
Standard Protocols
Click here to view our Standard Protocols