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验证数据展示

  • IF Staining of A431 using CL488-60320

    IF Staining of A431 using CL488-60320

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CL488-60320 (Cytokeratin 14 antibody) at dilution of 1:100.

  • IF Staining of mouse skin using CL488-60320

    IF Staining of mouse skin using CL488-60320

    Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using CoraLite®488 Cytokeratin 14 antibody (CL488-60320, Clone: 2G1E2 ) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite®488-conjugated Cytokeratin 14 Monoclonal antibody

Cytokeratin 14 Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL488-60320
CloneNo. 2G1E2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC, IF-P

Synonyms

KRT14, Cytokeratin-14, Cytokeratin,Keratin, CK-14, CK14

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®488
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse skin tissue
Positive IF/ICC detected inA431 cells, HepG2 cells, HaCaT cells
Planning an IHC experiment? We recommend our IHCeasy KRT14 Ready-To-Use IHC Kit. KRT14 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60320-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60320 targets Cytokeratin 14 in IF/ICC, IF-P applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IF-P Application Description
Tested Reactivity human, mouse
Immunogen Cytokeratin 14 fusion protein Ag17559 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name keratin 14
Synonyms KRT14, Cytokeratin-14, Cytokeratin,Keratin, CK-14, CK14
Calculated Molecular Weight 472 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC002690
Gene Symbol Cytokeratin 14
Gene ID (NCBI) 3861
RRIDAB_2934432
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths491 nm / 516 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02533
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

eratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.

实验方案

Product Specific Protocols
IF protocol for CL488 Cytokeratin 14 antibody CL488-60320Download protocol
Standard Protocols
Click here to view our Standard Protocols