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验证数据展示

  • IF Staining of mouse skin using CL647-60320

    IF Staining of mouse skin using CL647-60320

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using CoraLite® Plus 647 Cytokeratin 14 antibody (CL647-60320, Clone: 2G1E2 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse skin using CL647-60320

    IF Staining of mouse skin using CL647-60320

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using CoraLite® Plus 647 Cytokeratin 14 antibody (CL647-60320, Clone: 2G1E2 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of A431 using CL647-60320

    FC experiment of A431 using CL647-60320

    1X10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human Cytokeratin 14 (CL647-60320, Clone:2G1E2) (red), or 0.8 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21) (CL647-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated Cytokeratin 14 Monoclonal antibody

Cytokeratin 14 Monoclonal Antibody for IF-P, FC (Intra)
Cat No. CL647-60320

产品说明书

CloneNo. 2G1E2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF-P, FC (Intra)

别名

KRT14, Cytokeratin-14, Cytokeratin,Keratin, CK-14, CK14

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse skin tissue
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy KRT14 Ready-To-Use IHC Kit. KRT14 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60320-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-60320 targets Cytokeratin 14 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原 Cytokeratin 14 fusion protein Ag17559 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 keratin 14
别名 KRT14, Cytokeratin-14, Cytokeratin,Keratin, CK-14, CK14
计算分子量 472 aa, 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC002690
基因名称 Cytokeratin 14
Gene ID (NCBI) 3861
RRIDAB_2934987
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP02533
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Cytokeratin 14 antibody CL647-60320Download protocol
FC protocol for CL Plus 647 Cytokeratin 14 antibody CL647-60320Download protocol
Standard Protocols
Click here to view our Standard Protocols