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Cytokeratin 17-specific Recombinant antibody

Cytokeratin 17-specific Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242240A8

Cat no : 84978-1-RR

Print datasheet

Synonyms

Cytokeratin 17, KRT17, Cytokeratin-17, Cytokeratin,Keratin, CK-17

验证数据展示

  • WB analysis using 84978-1-RR

    WB analysis using 84978-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84978-1-RR (KRT17-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 84978-1-RR

    IHC staining of human skin cancer using 84978-1-RR

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84978-1-RR (Cytokeratin 17-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT17 antibody included
  • IHC staining of human skin cancer using 84978-1-RR

    IHC staining of human skin cancer using 84978-1-RR

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84978-1-RR (Cytokeratin 17-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT17 antibody included
  • IF Staining of HeLa using 84978-1-RR

    IF Staining of HeLa using 84978-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 17-Specific antibody (84978-1-RR, Clone: 242240A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, A431 cells, mouse skin tissue, rat skin tissue
Positive IHC detected inhuman skin cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, A431 cells
Planning an IHC experiment? We recommend our IHCeasy KRT17 Ready-To-Use IHC Kit. KRT17 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84978-1-RR targets Cytokeratin 17-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name keratin 17
Synonyms Cytokeratin 17, KRT17, Cytokeratin-17, Cytokeratin,Keratin, CK-17
Calculated Molecular Weight 48 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberNM_000422
Gene Symbol Cytokeratin 17
Gene ID (NCBI) 3872
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ04695
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filaments that make up the cytoskeleton. Type I keratins are a group of acidic intermediate filament proteins and type II keratins are the basic or neutral counterparts. Keratin 17 is encoded by the KRT17 gene and is a type I cytokeratin found in nail beds, hair follicles, sebaceous glands, and epidermal appendages.The molecular weight of cytokeratin 17 is approximately 49 kDa.Cytokeratin 17 plays a role in the formation and maintenance of epidermal appendages, especially in determining the shape and orientation of hair. It is required for the maintenance of the anagen (growth) state of hair follicles by modulating the function of TNF-alpha for hair cycling and is also involved in tissue repair (PMID: 16702408).

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 17-specific antibody 84978-1-RRDownload protocol
IHC protocol for Cytokeratin 17-specific antibody 84978-1-RRDownload protocol
IF protocol for Cytokeratin 17-specific antibody 84978-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols