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Cytokeratin 18 Polyclonal antibody, PBS Only

Cytokeratin 18 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 10830-1-PBS

Print datasheet

Synonyms

KRT18, keratin 18, Cytokeratin,Keratin, CK-18, CK18

验证数据展示

  • WB analysis using 10830-1-AP (same clone as 10830-1-PBS)

    WB analysis using 10830-1-AP (same clone as 10830-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 10830-1-AP (same clone as 10830-1-PBS)

    IHC staining of human breast cancer using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT18 antibody included
  • IHC staining of human breast cancer using 10830-1-AP (same clone as 10830-1-PBS)

    IHC staining of human breast cancer using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT18 antibody included
  • IF Staining of HepG2 using 10830-1-AP (same clone as 10830-1-PBS)

    IF Staining of HepG2 using 10830-1-AP (same clone as 10830-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cytokeratin 18 antibody (10830-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • IP experiment of A431 using 10830-1-AP (same clone as 10830-1-PBS)

    IP experiment of A431 using 10830-1-AP (same clone as 10830-1-PBS)

    IP result of anti-Cytokeratin 18 (IP:10830-1-AP, 3ug; Detection:10830-1-AP 1:2000) with A431 cells lysate 3300ug. This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate hyperplasia using 10830-1-AP (same clone as 10830-1-PBS)

    IHC staining of human prostate hyperplasia using 10830-1-AP (same clone as 10830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT18 antibody included
  • FC experiment of HeLa using 10830-1-AP (same clone as 10830-1-PBS)

    FC experiment of HeLa using 10830-1-AP (same clone as 10830-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Cytokeratin 18 Polyclonal antibody (10830-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy KRT18 Ready-To-Use IHC Kit. KRT18 primary antibody included.

产品信息

10830-1-PBS targets Cytokeratin 18 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Cytokeratin 18 fusion protein Ag1260 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 18
Synonyms KRT18, keratin 18, Cytokeratin,Keratin, CK-18, CK18
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC000180
Gene Symbol Cytokeratin 18
Gene ID (NCBI) 3875
RRIDAB_2133164
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05783
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.