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验证数据展示

  • WB analysis using 10830-1-AP

    WB analysis using 10830-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 10830-1-AP

    IHC staining of human breast cancer using 10830-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens).

    Ready-to-use IHC kits available with KRT18 antibody included
  • IHC staining of human breast cancer using 10830-1-AP

    IHC staining of human breast cancer using 10830-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens).

    Ready-to-use IHC kits available with KRT18 antibody included
  • IP experiment of A431 using 10830-1-AP

    IP experiment of A431 using 10830-1-AP

    IP result of anti-Cytokeratin 18 (IP:10830-1-AP, 3ug; Detection:10830-1-AP 1:2000) with A431 cells lysate 3300ug.

  • IF Staining of HepG2 using 10830-1-AP

    IF Staining of HepG2 using 10830-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cytokeratin 18 antibody (10830-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • FC experiment of HeLa using 10830-1-AP

    FC experiment of HeLa using 10830-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Cytokeratin 18 Polyclonal antibody (10830-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Cytokeratin 18 Polyclonal antibody

Cytokeratin 18 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 10830-1-AP

产品说明书

引用文献(116)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (6)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

别名

KRT18, Cell proliferation-inducing gene 46 protein, CK 18, CK18, CK-18

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, K-562 cells, MCF-7 cells, MDA-MB-231 cells, mouse liver tissue, rat liver tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman breast cancer tissue, human appendicitis tissue, human colon cancer tissue, human endometrial cancer tissue, human kidney tissue, human liver cancer tissue, human lung cancer tissue, human ovary tumor tissue, human pancreas cancer tissue, human prostate hyperplasia tissue, human renal cell carcinoma tissue, human stomach cancer tissue, human tonsillitis tissue, mouse liver tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT18 Ready-To-Use IHC Kit. KRT18 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10830-1-AP targets Cytokeratin 18 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, monkey, bovine, sheep, goat, duck
免疫原 Cytokeratin 18 fusion protein Ag1260 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 18
别名 KRT18, Cell proliferation-inducing gene 46 protein, CK 18, CK18, CK-18
计算分子量 48 kDa
观测分子量 48 kDa
GenBank蛋白编号BC000180
基因名称 Cytokeratin 18
Gene ID (NCBI) 3875
RRIDAB_2133164
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP05783
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 18 antibody 10830-1-APDownload protocol
IHC protocol for Cytokeratin 18 antibody 10830-1-APDownload protocol
IF protocol for Cytokeratin 18 antibody 10830-1-APDownload protocol
IP protocol for Cytokeratin 18 antibody 10830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Cell Stem Cell

Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency.

Authors - Brian Lin
View Article
mouseIF

Hepatology

Fat-associated Lymphoid Clusters as Expandable Niches for Ectopic Liver Development.

Authors - Bing Han
View Article
ratIHC

J Control Release

Mesenchymal stem cell exosomes as nanotherapeutics for dry age-related macular degeneration

Authors - Yue Tang
View Article
ratIF

Acta Biomater

A KPV-binding double-network hydrogel restores gut mucosal barrier in an inflamed colon.

Authors - Yingzheng Zhao
View Article
mouseIHC,IF

Proc Natl Acad Sci U S A

Role of estrogen receptor beta in uterine stroma and epithelium: Insights from estrogen receptor beta-/- mice.

Authors - Wada-Hiraike Osamu O
View Article
mouseIF

Proc Natl Acad Sci U S A

Basal epithelial stem cells are efficient targets for prostate cancer initiation.

Authors - Lawson Devon A DA
View Article