CoraLite® Plus 488-conjugated Cytokeratin 18 Polyclonal antibody

Cytokeratin 18 Polyclonal Antibody for WB, IF/ICC, FC (Intra)
Cat No. CL488-10830

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra)

CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inA431 cells, HeLa cells, K-562 cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT18 Ready-To-Use IHC Kit. KRT18 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10830-1-AP

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10830 targets Cytokeratin 18 in WB, IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 Cytokeratin 18 fusion protein Ag1260 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 18
别名 CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18
计算分子量 48 kDa
观测分子量 48 kDa
GenBank蛋白编号BC000180
基因名称 Cytokeratin 18
Gene ID (NCBI) 3875
RRIDAB_2918999
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP05783
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 Cytokeratin 18 antibody CL488-10830Download protocol
IF protocol for CL Plus 488 Cytokeratin 18 antibody CL488-10830Download protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...