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CoraLite®555-conjugated Cytokeratin 7 Polyclonal antibody

Cytokeratin 7 Polyclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-15539

Print datasheet

Synonyms

KRT7, keratin 7, Cytokeratin-7, Cytokeratin,Keratin, CK-7

验证数据展示

  • IF Staining of human breast cancer using CL555-15539

    IF Staining of human breast cancer using CL555-15539

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 Cytokeratin 7 antibody (CL555-15539) at dilution of 1:800, CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11, Magenta), CoraLite®488 CD31 antibody (CL488-11265, green).

  • IF Staining of HeLa using CL555-15539

    IF Staining of HeLa using CL555-15539

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®555 Cytokeratin 7 antibody (CL555-15539) at dilution of 1:200.

  • IF Staining of HepG2 using CL555-15539

    IF Staining of HepG2 using CL555-15539

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®555 Cytokeratin 7 antibody (CL555-15539) at dilution of 1:200.

  • FC experiment of HeLa using CL555-15539

    FC experiment of HeLa using CL555-15539

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®555-conjugated Cytokeratin 7 Polyclonal antibody (CL555-15539)(red), or 0.8 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF-P detected inhuman breast cancer tissue
Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Cytokeratin 7 Ready-To-Use IHC Kit. Cytokeratin 7 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15539-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-15539 targets Cytokeratin 7 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Cytokeratin 7 fusion protein Ag7895 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 7
Synonyms KRT7, keratin 7, Cytokeratin-7, Cytokeratin,Keratin, CK-7
Calculated Molecular Weight 469 aa, 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC002700
Gene Symbol Cytokeratin 7
Gene ID (NCBI) 3855
RRIDAB_2919627
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08729
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.

实验方案

Product Specific Protocols
IF protocol for CL555 Cytokeratin 7 antibody CL555-15539Download protocol
Standard Protocols
Click here to view our Standard Protocols