验证数据展示
经过测试的应用
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-15539 targets Cytokeratin 7 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
经测试应用 | FC (Intra) Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | Cytokeratin 7 fusion protein Ag7895 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | keratin 7 |
别名 | KRT7, keratin 7, Cytokeratin-7, Cytokeratin,Keratin, CK-7 |
计算分子量 | 469 aa, 51 kDa |
观测分子量 | 51 kDa |
GenBank蛋白编号 | BC002700 |
基因名称 | Cytokeratin 7 |
Gene ID (NCBI) | 3855 |
RRID | AB_2934917 |
偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
最大激发/发射波长 | 654 nm / 674 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P08729 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.
实验方案
Product Specific Protocols | |
---|---|
FC protocol for CL Plus 647 Cytokeratin 7 antibody CL647-15539 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |