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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated Cytokeratin 7 Monoclonal antibody

Cytokeratin 7 Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2E1G5

Cat no : CL555-66483

Print datasheet

Synonyms

KRT7, Cytokeratin-7, Cytokeratin,Keratin, CK-7, CK7

验证数据展示

  • IF Staining of human prostate cancer using CL555-66483

    IF Staining of human prostate cancer using CL555-66483

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite®555 Cytokeratin 7 antibody (CL555-66483, Clone: 2E1G5 ) at dilution of 1:200.

  • IF Staining of human prostate cancer using CL555-66483

    IF Staining of human prostate cancer using CL555-66483

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite®555 Cytokeratin 7 antibody (CL555-66483, Clone: 2E1G5 ) at dilution of 1:200.

  • FC experiment of HeLa using CL555-66483

    FC experiment of HeLa using CL555-66483

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug CoraLite®555 Anti-Human Cytokeratin 7 (CL555-66483, Clone:2E1G5) (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF-P detected inhuman prostate cancer tissue
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Cytokeratin 7 Ready-To-Use IHC Kit. Cytokeratin 7 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66483-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66483 targets Cytokeratin 7 in IF-P, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity human
Immunogen Cytokeratin 7 fusion protein Ag7895 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name keratin 7
Synonyms KRT7, Cytokeratin-7, Cytokeratin,Keratin, CK-7, CK7
Calculated Molecular Weight 469 aa, 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC002700
Gene Symbol Cytokeratin 7
Gene ID (NCBI) 3855
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08729
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7, also named as cytokeratin 7, is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels, and their neoplasms. KRT7 is marker of epithelial tissues, but not present in carcinomas of stratified squamous cell origin.This antibody is specifically against KRT7.

实验方案

Product Specific Protocols
IF protocol for CL555 Cytokeratin 7 antibody CL555-66483Download protocol
FC protocol for CL555 Cytokeratin 7 antibody CL555-66483Download protocol
Standard Protocols
Click here to view our Standard Protocols