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验证数据展示

  • IF Staining of human lung cancer using CL594-66483

    IF Staining of human lung cancer using CL594-66483

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CL594-66483 (Cytokeratin 7 antibody) at dilution of 1:50 .

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated Cytokeratin 7 Monoclonal antibody

Cytokeratin 7 Monoclonal Antibody for IF-P
Cat No. CL594-66483

产品说明书

CloneNo. 2E1G5

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

IF-P

别名

Cell and organelle markers, CK 7, CK7, Cytokeratin 7, Cytoskeleton Marker, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman lung cancer tissue, human prostate cancer tissue
Planning an IHC experiment? We recommend our IHCeasy Cytokeratin 7 Ready-To-Use IHC Kit. Cytokeratin 7 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66483-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66483 targets Cytokeratin 7 in IF-P applications and shows reactivity with Human samples.

经测试应用 IF-P Application Description
经测试反应性 Human
免疫原 Cytokeratin 7 fusion protein Ag7895 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 keratin 7
别名 Cell and organelle markers, CK 7, CK7, Cytokeratin 7, Cytoskeleton Marker, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7
计算分子量 469 aa, 51 kDa
GenBank蛋白编号BC002700
基因名称 Cytokeratin 7
Gene ID (NCBI) 3855
RRIDAB_2883589
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08729
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7, also named as cytokeratin 7, is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels, and their neoplasms. KRT7 is marker of epithelial tissues, but not present in carcinomas of stratified squamous cell origin.This antibody is specifically against KRT7. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

实验方案

Product Specific Protocols
IF protocol for CL594 Cytokeratin 7 antibody CL594-66483Download protocol
Standard Protocols
Click here to view our Standard Protocols