Drebrin Polyclonal antibody

Drebrin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Publications

Cat no : 10260-1-AP

Print datasheet

Synonyms

DBN1, drebrin 1, Developmentally-regulated brain protein, DBN 1

验证数据展示

WB analysis of HeLa using 10260-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10260-1-AP (DBN1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 10260-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10260-1-AP (Drebrin antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using 10260-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Drebrin antibody (10260-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IP experiment of K-562 using 10260-1-AP
IP result of anti-Drebrin (IP:10260-1-AP, 4ug; Detection:10260-1-AP 1:2000) with K-562 cells lysate 1200ug.
IHC staining of mouse testis using 10260-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10260-1-AP (Drebrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with DBN1/Drebrin antibody included
IHC staining of mouse testis using 10260-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10260-1-AP (Drebrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with DBN1/Drebrin antibody included
IF Staining of HepG2 using 10260-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Drebrin antibody (10260-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 10260-1-AP

Size

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经过测试的应用

Positive WB detected in	HeLa cells, K-562 cells, Neuro-2a cells
Positive IP detected in	K-562 cells
Positive IHC detected in	mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells

Planning an IHC experiment? We recommend our IHCeasy DBN1/Drebrin Ready-To-Use IHC Kit. DBN1/Drebrin primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 18 publications below
IHC	See 4 publications below
IF	See 4 publications below
IP	See 1 publications below

产品信息

10260-1-AP targets Drebrin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications	WB, IHC, IF/ICC, IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP
Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Immunogen	Drebrin fusion protein Ag0371 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	drebrin 1
Synonyms	DBN1, drebrin 1, Developmentally-regulated brain protein, DBN 1
Calculated Molecular Weight	71 kDa
Observed Molecular Weight	100-130 kDa
GenBank Accession Number	BC000283
Gene Symbol	Drebrin
Gene ID (NCBI)	1627
ENSEMBL Gene ID	ENSG00000113758
RRID	AB_2230301
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16643
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.

实验方案

Product Specific Protocols
WB protocol for Drebrin antibody 10260-1-AP	Download protocol
IHC protocol for Drebrin antibody 10260-1-AP	Download protocol
IF protocol for Drebrin antibody 10260-1-AP	Download protocol
IP protocol for Drebrin antibody 10260-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	IF	Dev Cell An integrated approach identifies the molecular underpinnings of murine anterior visceral endoderm migration Authors - Shifaan Thowfeequ View Article
mouse	IF	PLoS Genet N-wasp is required for structural integrity of the blood-testis barrier. Authors - Xiang Xiao View Article
human	IF	Int J Mol Sci Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery Authors - Paloma Alvarez-Suarez View Article
human	WB	Oncogenesis Tumor suppressor BAP1 suppresses disulfidptosis through the regulation of SLC7A11 and NADPH levels Authors - Jin Wang View Article
human	WB	Biol Direct Glucose deprivation-induced disulfidptosis in human nucleus pulposus cells: a novel pathological mechanism of intervertebral disc degeneration Authors - Shaobo Wu View Article
mouse,rat	WB	Mol Neurobiol L-3-n-Butylphthalide improves synaptic and dendritic spine plasticity and ameliorates neurite pathology in Alzheimer's disease mouse model and cultured hippocampal neurons. Authors - Longjian Huang View Article

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Drebrin Polyclonal antibody

Drebrin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

验证数据展示

WB analysis of HeLa using 10260-1-AP

WB analysis using 10260-1-AP

IF Staining of HeLa using 10260-1-AP

IP experiment of K-562 using 10260-1-AP

IHC staining of mouse testis using 10260-1-AP

IHC staining of mouse testis using 10260-1-AP

IF Staining of HepG2 using 10260-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

An integrated approach identifies the molecular underpinnings of murine anterior visceral endoderm migration

N-wasp is required for structural integrity of the blood-testis barrier.

Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery

Tumor suppressor BAP1 suppresses disulfidptosis through the regulation of SLC7A11 and NADPH levels

Glucose deprivation-induced disulfidptosis in human nucleus pulposus cells: a novel pathological mechanism of intervertebral disc degeneration

L-3-n-Butylphthalide improves synaptic and dendritic spine plasticity and ameliorates neurite pathology in Alzheimer's disease mouse model and cultured hippocampal neurons.