验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, K-562 cells, Neuro-2a cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10260-1-AP targets Drebrin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Drebrin fusion protein Ag0371 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | drebrin 1 |
| 别名 | DBN1, drebrin 1, Developmentally-regulated brain protein, DBN 1 |
| 计算分子量 | 71 kDa |
| 观测分子量 | 100-130 kDa |
| GenBank蛋白编号 | BC000283 |
| 基因名称 | Drebrin |
| Gene ID (NCBI) | 1627 |
| ENSEMBL Gene ID | ENSG00000113758 |
| RRID | AB_2230301 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16643 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Drebrin antibody 10260-1-AP | Download protocol |
| IHC protocol for Drebrin antibody 10260-1-AP | Download protocol |
| IF protocol for Drebrin antibody 10260-1-AP | Download protocol |
| IP protocol for Drebrin antibody 10260-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Dev Cell An integrated approach identifies the molecular underpinnings of murine anterior visceral endoderm migration | ||
Int J Mol Sci Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery | ||
Oncogenesis Tumor suppressor BAP1 suppresses disulfidptosis through the regulation of SLC7A11 and NADPH levels | ||
Biol Direct Glucose deprivation-induced disulfidptosis in human nucleus pulposus cells: a novel pathological mechanism of intervertebral disc degeneration | ||
Mol Neurobiol L-3-n-Butylphthalide improves synaptic and dendritic spine plasticity and ameliorates neurite pathology in Alzheimer's disease mouse model and cultured hippocampal neurons. |
