CoraLite® Plus 488-conjugated Drebrin Polyclonal antibody
Drebrin Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy DBN1/Drebrin Ready-To-Use IHC Kit. DBN1/Drebrin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10260-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-10260 targets Drebrin in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | Drebrin fusion protein Ag0371 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | drebrin 1 |
| Synonyms | D0S117E, DBN1, DKFZp434D064, Drebrin, drebrin 1 |
| Calculated Molecular Weight | 71 kDa |
| Observed Molecular Weight | 100-130 kDa |
| GenBank Accession Number | BC000283 |
| Gene Symbol | Drebrin |
| Gene ID (NCBI) | 1627 |
| ENSEMBL Gene ID | ENSG00000113758 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16643 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Drebrin antibody CL488-10260 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |