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CoraLite® Plus 488-conjugated DCLK1 Polyclonal antibody

DCLK1 Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-29800

Print datasheet

Synonyms

DCAMKL1, DCDC3A, DCLK, DCLK1, doublecortin like kinase 1, KIAA0369

验证数据展示

  • IF Staining of mouse brain using CL488-29800

    IF Staining of mouse brain using CL488-29800

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 DCLK1 antibody (CL488-29800) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using CL488-29800

    IF Staining of mouse brain using CL488-29800

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 DCLK1 antibody (CL488-29800) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of Neuro-2a using CL488-29800

    FC experiment of Neuro-2a using CL488-29800

    1x10^6 Neuro-2a cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Dclk1 Polyclonal Antibody (CL488-29800)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inNeuro-2a cells
Positive FC detected inNeuro-2a cells
For other applications, we recommend the unconjugated version of this antibody, 29800-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-29800 targets DCLK1 in IF-P, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity Human, mouse, rat
Immunogen DCLK1 fusion protein Ag32007 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name doublecortin-like kinase 1
Synonyms DCAMKL1, DCDC3A, DCLK, DCLK1, doublecortin like kinase 1, KIAA0369
Calculated Molecular Weight82KD
Observed Molecular Weight48 kDa, 82 kDa
GenBank Accession NumberNM_004734
Gene Symbol DCLK1
Gene ID (NCBI) 9201
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15075
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCLK1 (Serine/threonine-protein kinase DCLK1) is also named as DCAMKL1, DCDC3A, KIAA0369 and belongs to the CAMK Ser/Thr protein kinase family. It is a microtubule-associated kinase that can undergo autophosphorylation and it also has microtubule-polymerizing activity that is independent of its protein kinase activity (PMID: 11124993). It plays a unique role in mitotic spindle integrity during early neurogenesis in radial glial cell proliferation and their radial process stability. DCLK1 is a unique marker for distinguishing tumor stem cells from intestinal normal stem cells (PMID: 23202126). This protein has 4 isoforms produced by alternative splicing with the molecular weight of 82 kDa, 81 kDa, 47 kDa and 48 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DCLK1 antibody CL488-29800Download protocol
Standard Protocols
Click here to view our Standard Protocols