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验证数据展示

  • IF Staining of HepG2 using CL488-21445

    IF Staining of HepG2 using CL488-21445

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 DDRGK1 antibody (CL488-21445) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-21445

    FC experiment of HepG2 using CL488-21445

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated DDRGK1 Polyclonal antibody (CL488-21445)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated DDRGK1 Polyclonal antibody

DDRGK1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-21445

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

UFM1-binding and PCI domain-containing protein 1, UFBP1, DDRGK domain-containing protein 1, DDRGK domain containing 1, Dashurin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 21445-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21445 targets DDRGK1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 DDRGK1 fusion protein Ag13742 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DDRGK domain containing 1
别名 UFM1-binding and PCI domain-containing protein 1, UFBP1, DDRGK domain-containing protein 1, DDRGK domain containing 1, Dashurin
计算分子量 314 aa, 36 kDa
观测分子量 36-46 kDa
GenBank蛋白编号BC000643
基因名称 DDRGK1
Gene ID (NCBI) 65992
RRIDAB_3672732
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96HY6
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDRGK1, also name as C20orf116, is a member of the DDRGK domain-containing protein family with unknown function. Depletion of DDRGK1 inhibits cell proliferation and invasion. Expression of NF-κB target genes showed the most significant decrease after depleting of DDRGK1, suggesting that DDRGK1 may play an important role in the NF-κB signaling pathway. DDRGK1 interacts with IκBα and regulates its stability, thereby regulates the NF-κB transcriptional activity. DDRGK1 acts as an important regulator of the NF-κB pathway. (PMID: 23675531) Catalog#21445-1-AP is a rabbit polyclonal antibody raised against the full-length of human DDRGK1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DDRGK1 antibody CL488-21445Download protocol
FC protocol for CL Plus 488 DDRGK1 antibody CL488-21445Download protocol
Standard Protocols
Click here to view our Standard Protocols