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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DDRGK1 Polyclonal antibody

DDRGK1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21445

Print datasheet

Synonyms

UFM1-binding and PCI domain-containing protein 1, UFBP1, DDRGK domain-containing protein 1, DDRGK domain containing 1, Dashurin

验证数据展示

  • IF Staining of HepG2 using CL488-21445

    IF Staining of HepG2 using CL488-21445

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 DDRGK1 antibody (CL488-21445) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-21445

    FC experiment of HepG2 using CL488-21445

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated DDRGK1 Polyclonal antibody (CL488-21445)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 21445-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21445 targets DDRGK1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen DDRGK1 fusion protein Ag13742 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DDRGK domain containing 1
Synonyms UFM1-binding and PCI domain-containing protein 1, UFBP1, DDRGK domain-containing protein 1, DDRGK domain containing 1, Dashurin
Calculated Molecular Weight 314 aa, 36 kDa
Observed Molecular Weight 36-46 kDa
GenBank Accession NumberBC000643
Gene Symbol DDRGK1
Gene ID (NCBI) 65992
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96HY6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDRGK1, also name as C20orf116, is a member of the DDRGK domain-containing protein family with unknown function. Depletion of DDRGK1 inhibits cell proliferation and invasion. Expression of NF-κB target genes showed the most significant decrease after depleting of DDRGK1, suggesting that DDRGK1 may play an important role in the NF-κB signaling pathway. DDRGK1 interacts with IκBα and regulates its stability, thereby regulates the NF-κB transcriptional activity. DDRGK1 acts as an important regulator of the NF-κB pathway. (PMID: 23675531) Catalog#21445-1-AP is a rabbit polyclonal antibody raised against the full-length of human DDRGK1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DDRGK1 antibody CL488-21445Download protocol
FC protocol for CL Plus 488 DDRGK1 antibody CL488-21445Download protocol
Standard Protocols
Click here to view our Standard Protocols