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验证数据展示

  • WB analysis using 11324-1-AP (same clone as 11324-1-PBS)

    WB analysis using 11324-1-AP (same clone as 11324-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11324-1-AP (DDX20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11324-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 11324-1-AP (same clone as 11324-1-PBS)

    FC experiment of HepG2 using 11324-1-AP (same clone as 11324-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human DDX20 (11324-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11324-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 11324-1-AP (same clone as 11324-1-PBS)

    IF Staining of HepG2 using 11324-1-AP (same clone as 11324-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using DDX20 antibody 11324-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 11324-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 11324-1-AP (same clone as 11324-1-PBS)

    IP experiment of HeLa using 11324-1-AP (same clone as 11324-1-PBS)

    IP result of anti-DDX20 (IP:11324-1-AP, 4ug; Detection:11324-1-AP 1:2000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 11324-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 11324-1-AP (same clone as 11324-1-PBS)

    IHC staining of human breast cancer using 11324-1-AP (same clone as 11324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11324-1-AP (DDX20 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11324-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDX20 antibody included
  • Featured Product
  • KD/KO验证

DDX20 Polyclonal antibody, PBS Only

DDX20 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 11324-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy DDX20 Ready-To-Use IHC Kit. DDX20 primary antibody included.

产品信息

11324-1-PBS targets DDX20 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 DDX20 fusion protein Ag1863 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
别名
计算分子量 824 aa, 92 kDa
观测分子量 100 kDa
GenBank蛋白编号BC011556
基因名称 DDX20
Gene ID (NCBI) 11218
RRIDAB_2092401
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHI6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (DDX20), also known as DP103 or Gemin3, is a member of the DEAD box protein family expressed ubiquitously. DEAD family proteins use energy from ATP hydrolysis for RNA chaperoning and RNPase activity (PMID: 27121695). As a core member of the survival motor neuron (SMN) complex, DDX20 participate in small nuclear ribonucleoprotein (snRNP) biogenesis. Second, DDX20 have direct roles in gene expression in view of its implication in transcription and post-transcriptional gene silencing. Addition, the false expression of DDX20 could have deleterious effects on cellular homeostasis thus leading to cancer development and progression (PMID:29523774). Anymore, DDX20 could be identified as a biomarker and metastasis-driving oncogene of human breast cancer (PMID: 25083991). The detected weight of DDX20 is slightly higher than the theoretical molecular weight that is because of phosphorylation after translation.