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DDX21 Monoclonal antibody

DDX21 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1B9C8

Cat no : 66925-1-Ig

Print datasheet

Synonyms

GUA, Gu alpha, EC:3.6.4.13, DExD-box helicase 21, DEAD box protein 21

验证数据展示

  • WB analysis using 66925-1-Ig

    WB analysis using 66925-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66925-1-Ig (DDX21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66925-1-Ig

    WB analysis using 66925-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66925-1-Ig (DDX21 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IHC staining of human colon cancer using 66925-1-Ig

    IHC staining of human colon cancer using 66925-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66925-1-Ig (DDX21 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 66925-1-Ig

    IF Staining of HepG2 using 66925-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX21 antibody (66925-1-Ig, Clone: 1B9C8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 66925-1-Ig

    FC experiment of HepG2 using 66925-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human DDX21 (66925-1-Ig, Clone:1B9C8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HL-60 cells, THP-1 cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66925-1-Ig targets DDX21 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC, IP
Tested Reactivity human
Cited Reactivityhuman
Immunogen DDX21 fusion protein Ag17850 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
Synonyms GUA, Gu alpha, EC:3.6.4.13, DExD-box helicase 21, DEAD box protein 21
Calculated Molecular Weight 783 aa, 87 kDa
Observed Molecular Weight 90-95 kDa
GenBank Accession NumberBC008071
Gene Symbol DDX21
Gene ID (NCBI) 9188
RRIDAB_2882252
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NR30
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX21 protein belongs to DEAD box protein family which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). As a putative RNA helicase, DDX21 unwinds double-stranded RNA, folds single-stranded RNA and is involved in process including ribosomal RNA biogeneis, RNA editing and general transcription. Interaction of DDX21 and c-Jun was reported in ribosomal RNA processing.

实验方案

Product Specific Protocols
WB protocol for DDX21 antibody 66925-1-IgDownload protocol
IHC protocol for DDX21 antibody 66925-1-IgDownload protocol
IF protocol for DDX21 antibody 66925-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP,IHC

Cell Death Dis

TRIP13 regulates progression of gastric cancer through stabilising the expression of DDX21

Authors - Guanghui Zhang
View Article