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CoraLite®594-conjugated DDX21 Monoclonal antibody

DDX21 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B9C8

Cat no : CL594-66925

Print datasheet

Synonyms

GUA, Gu alpha, EC:3.6.4.13, DExD-box helicase 21, DEAD box protein 21

验证数据展示

  • IF Staining of HepG2 using CL594-66925

    IF Staining of HepG2 using CL594-66925

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594-conjugated DDX21 antibody (CL594-66925, Clone: 1B9C8 ) at dilution of 1:100.

  • FC experiment of HepG2 using CL594-66925

    FC experiment of HepG2 using CL594-66925

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human DDX21 (CL594-66925, Clone:1B9C8) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66925-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66925 targets DDX21 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen DDX21 fusion protein Ag17850 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
Synonyms GUA, Gu alpha, EC:3.6.4.13, DExD-box helicase 21, DEAD box protein 21
Calculated Molecular Weight 783 aa, 87 kDa
GenBank Accession NumberBC008071
Gene Symbol DDX21
Gene ID (NCBI) 9188
RRIDAB_2920058
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NR30
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX21 protein belongs to DEAD box protein family which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). As a putative RNA helicase, DDX21 unwinds double-stranded RNA, folds single-stranded RNA and is involved in process including ribosomal RNA biogeneis, RNA editing and general transcription. Interaction of DDX21 and c-Jun was reported in ribosomal RNA processing.

实验方案

Product Specific Protocols
IF protocol for CL594 DDX21 antibody CL594-66925Download protocol
FC protocol for CL594 DDX21 antibody CL594-66925Download protocol
Standard Protocols
Click here to view our Standard Protocols