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验证数据展示

  • IF Staining of HepG2 using CL594-66925

    IF Staining of HepG2 using CL594-66925

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594-conjugated DDX21 antibody (CL594-66925, Clone: 1B9C8 ) at dilution of 1:100.

  • FC experiment of HepG2 using CL594-66925

    FC experiment of HepG2 using CL594-66925

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human DDX21 (CL594-66925, Clone:1B9C8) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite®594-conjugated DDX21 Monoclonal antibody

DDX21 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66925

产品说明书

CloneNo. 1B9C8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

GUA, Gu alpha, EC:3.6.4.13, DExD-box helicase 21, DEAD box protein 21

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66925-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66925 targets DDX21 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 DDX21 fusion protein Ag17850 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
别名 GUA, Gu alpha, EC:3.6.4.13, DExD-box helicase 21, DEAD box protein 21
计算分子量 783 aa, 87 kDa
GenBank蛋白编号BC008071
基因名称 DDX21
Gene ID (NCBI) 9188
RRIDAB_2920058
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9NR30
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX21 protein belongs to DEAD box protein family which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). As a putative RNA helicase, DDX21 unwinds double-stranded RNA, folds single-stranded RNA and is involved in process including ribosomal RNA biogeneis, RNA editing and general transcription. Interaction of DDX21 and c-Jun was reported in ribosomal RNA processing.

实验方案

Product Specific Protocols
IF protocol for CL594 DDX21 antibody CL594-66925Download protocol
FC protocol for CL594 DDX21 antibody CL594-66925Download protocol
Standard Protocols
Click here to view our Standard Protocols