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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DENR Polyclonal antibody

DENR Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10656

Print datasheet

Synonyms

SMAP 3, Protein DRP1, H14, DRP1, DRP

验证数据展示

  • IF Staining of HEK-293 using CL488-10656

    IF Staining of HEK-293 using CL488-10656

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 DENR antibody (CL488-10656) at dilution of 1:200.

  • FC experiment of HEK-293 using CL488-10656

    FC experiment of HEK-293 using CL488-10656

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 DENR Polyclonal Antibody (CL488-10656)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 10656-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10656 targets DENR in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen DENR fusion protein Ag1023 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name density-regulated protein
Synonyms SMAP 3, Protein DRP1, H14, DRP1, DRP
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 27-30 kDa
GenBank Accession NumberBC007860
Gene Symbol DENR
Gene ID (NCBI) 8562
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43583
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DENR, also named as SMAP-3 or DRP1, is a 198 amino acid protein, which is highly expressed in heart and skeletal muscle and moderately expressed in the brain, placenta, liver and pancreas. DENR is Up-regulated with increasing cell-density by HNRNPD and Up-regulated in ovarian and breast cancer cells by ERBB2 overexpression. The calculated molecular weight of DENR is 22 kDa, but the modified protein is about 25-30 kDa. (PMID: 27239039 )

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DENR antibody CL488-10656Download protocol
FC protocol for CL Plus 488 DENR antibody CL488-10656Download protocol
Standard Protocols
Click here to view our Standard Protocols