验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A375 cells |
For other applications, we recommend the unconjugated version of this antibody, 66894-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66894 targets DLC1 in IF/ICC applications and shows reactivity with Human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human |
| 免疫原 | DLC1 fusion protein Ag24404 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | deleted in liver cancer 1 |
| 别名 | ARHGAP7, deleted in liver cancer 1, DLC 1, DLC1, FLJ21120, HP, HP protein, KIAA1723, p122 RhoGAP, STARD12 |
| 计算分子量 | 171 kDa |
| 观测分子量 | 115 kDa |
| GenBank蛋白编号 | BC054511 |
| 基因名称 | DLC1 |
| Gene ID (NCBI) | 10395 |
| RRID | AB_2934504 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q96QB1 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Deleted in Liver Cancer 1 (DLC1) is a RhoGAP-containing tumor suppressor that inhibits angiogenesis by repressing VEGF production in epithelial cells. DLC1 is often down-regulated in a variety of cancers, such as those of the liver, lung, breast, stomach, brain, colon and prostate due to either genomic deletion or aberrant DNA methylation.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 DLC1 antibody CL488-66894 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |