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CoraLite®594-conjugated DLC1 Monoclonal antibody

DLC1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G10H12

Cat no : CL594-66894

Print datasheet

Synonyms

ARHGAP7, deleted in liver cancer 1, DLC 1, DLC1, FLJ21120, HP, HP protein, KIAA1723, p122 RhoGAP, STARD12

验证数据展示

  • IF Staining of A375 using CL594-66894

    IF Staining of A375 using CL594-66894

    Immunofluorescent analysis of (-20°C Methanol) fixed A375 cells using CoraLite®594 DLC1 antibody (CL594-66894, Clone: 1G10H12 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inA375 cells
For other applications, we recommend the unconjugated version of this antibody, 66894-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66894 targets DLC1 in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen DLC1 fusion protein Ag24404 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name deleted in liver cancer 1
Synonyms ARHGAP7, deleted in liver cancer 1, DLC 1, DLC1, FLJ21120, HP, HP protein, KIAA1723, p122 RhoGAP, STARD12
Calculated Molecular Weight 171 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC054511
Gene Symbol DLC1
Gene ID (NCBI) 10395
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96QB1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Deleted in Liver Cancer 1 (DLC1) is a RhoGAP-containing tumor suppressor that inhibits angiogenesis by repressing VEGF production in epithelial cells. DLC1 is often down-regulated in a variety of cancers, such as those of the liver, lung, breast, stomach, brain, colon and prostate due to either genomic deletion or aberrant DNA methylation.

实验方案

Product Specific Protocols
IF protocol for CL594 DLC1 antibody CL594-66894Download protocol
Standard Protocols
Click here to view our Standard Protocols