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验证数据展示

  • IF Staining of mouse brain using CL647-25535

    IF Staining of mouse brain using CL647-25535

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 DLL3 antibody (CL647-25535) at dilution of 1:400.

  • IF Staining of mouse brain using CL647-25535

    IF Staining of mouse brain using CL647-25535

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 DLL3 antibody (CL647-25535) at dilution of 1:400.

CoraLite® Plus 647-conjugated DLL3 Polyclonal antibody

DLL3 Polyclonal Antibody for IF-P
Cat No. CL647-25535

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF-P

别名

delta like 3 (Drosophila), Delta like protein 3, Delta3, DLL3, Drosophila Delta homolog 3, SCDO1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 25535-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-25535 targets DLL3 in IF-P applications and shows reactivity with human, mouse samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse
免疫原 DLL3 fusion protein Ag21965 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 delta-like 3 (Drosophila)
别名 delta like 3 (Drosophila), Delta like protein 3, Delta3, DLL3, Drosophila Delta homolog 3, SCDO1
计算分子量 65 kDa
观测分子量 65 kDa
GenBank蛋白编号BC000218
基因名称 DLL3
Gene ID (NCBI) 10683
RRIDAB_3084804
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NYJ7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Delta-Notch pathway is an evolutionarily conserved signaling pathway which controls a broad range of developmental processes including cell fate determination, terminal differentiation and proliferation (PMID: 22353464). In mammals, four Notch receptors (NOTCH1-4) and five activating canonical ligands (JAGGED1, JAGGED2, DLL1, DLL3 and DLL4) have been described (PMID: 22353464). DLL3 is an inhibitory ligand of the Notch signaling pathway that is predominantly localizes to the Golgi apparatus (PMID: 17664336) in normal condition. Normal tissue expression of DLL3 is highest in fetal brain, and DLL3 plays a key role in somitogenesis in the paraxial mesoderm (PMID: 26311731). It has been reported that DLL3 is expressed on the surface of tumor cells of small cell lung cancer (SCLC) and high-grade neuroendocrine carcinomas (LCNEC) and has emerged as a novel therapeutic target (PMID: 26311731; 28487384).

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 DLL3 antibody CL647-25535Download protocol
Standard Protocols
Click here to view our Standard Protocols