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CoraLite® Plus 647-conjugated DLL3 Polyclonal antibody

DLL3 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat No : CL647-25535

Print datasheet

Synonyms

delta like 3 (Drosophila), Delta like protein 3, Delta3, DLL3, Drosophila Delta homolog 3, SCDO1

验证数据展示

  • IF Staining of mouse brain using CL647-25535

    IF Staining of mouse brain using CL647-25535

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 DLL3 antibody (CL647-25535) at dilution of 1:400.

  • IF Staining of mouse brain using CL647-25535

    IF Staining of mouse brain using CL647-25535

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 DLL3 antibody (CL647-25535) at dilution of 1:400.

经过测试的应用

Positive IF-P detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 25535-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-25535 targets DLL3 in IF-P applications and shows reactivity with human, mouse samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse
Immunogen DLL3 fusion protein Ag21965 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name delta-like 3 (Drosophila)
Synonyms delta like 3 (Drosophila), Delta like protein 3, Delta3, DLL3, Drosophila Delta homolog 3, SCDO1
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC000218
Gene Symbol DLL3
Gene ID (NCBI) 10683
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NYJ7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Delta-Notch pathway is an evolutionarily conserved signaling pathway which controls a broad range of developmental processes including cell fate determination, terminal differentiation and proliferation (PMID: 22353464). In mammals, four Notch receptors (NOTCH1-4) and five activating canonical ligands (JAGGED1, JAGGED2, DLL1, DLL3 and DLL4) have been described (PMID: 22353464). DLL3 is an inhibitory ligand of the Notch signaling pathway that is predominantly localizes to the Golgi apparatus (PMID: 17664336) in normal condition. Normal tissue expression of DLL3 is highest in fetal brain, and DLL3 plays a key role in somitogenesis in the paraxial mesoderm (PMID: 26311731). It has been reported that DLL3 is expressed on the surface of tumor cells of small cell lung cancer (SCLC) and high-grade neuroendocrine carcinomas (LCNEC) and has emerged as a novel therapeutic target (PMID: 26311731; 28487384).

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 DLL3 antibody CL647-25535Download protocol
Standard Protocols
Click here to view our Standard Protocols