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CoraLite® Plus 488-conjugated DMGDH Polyclonal antibody

DMGDH Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-24813

Print datasheet

Synonyms

ME2GLYDH, M2GD, EC:1.5.8.4, DMGDHD, dimethylglycine dehydrogenase

验证数据展示

  • FC experiment of HepG2 using CL488-24813

    FC experiment of HepG2 using CL488-24813

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 DMGDH Polyclonal Antibody (CL488-24813)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 24813-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-24813 targets DMGDH in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen DMGDH fusion protein Ag20529 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dimethylglycine dehydrogenase
Synonyms ME2GLYDH, M2GD, EC:1.5.8.4, DMGDHD, dimethylglycine dehydrogenase
Calculated Molecular Weight 866 aa, 97 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC022388
Gene Symbol DMGDH
Gene ID (NCBI) 29958
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UI17
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DMGDH(Dimethylglycine dehydrogenase, mitochondrial) is also named as ME2GLYDH and belongs to the GcvT family. It catalyzes the oxidative demethylation of dimethylglycine to form sarcosine and it plays a role in choline catabolism in the liver. The full length protein has a transit peptide.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 DMGDH antibody CL488-24813Download protocol
Standard Protocols
Click here to view our Standard Protocols