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验证数据展示

  • FC experiment of Jurkat using CL488-66587

    FC experiment of Jurkat using CL488-66587

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human DNAJB6 (CL488-66587, Clone:1A1D2) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  • KD/KO验证

CoraLite® Plus 488-conjugated DNAJB6 Monoclonal antibody

DNAJB6 Monoclonal Antibody for FC (Intra)
Cat No. CL488-66587

产品说明书

CloneNo. 1A1D2

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Pig

应用

FC (Intra)

别名

HSJ2, HSJ 2, HHDJ1, Heat shock protein J2, DnaJ

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 66587-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66587 targets DNAJB6 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原 DNAJB6 fusion protein Ag2258 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily B, member 6
别名 HSJ2, HSJ 2, HHDJ1, Heat shock protein J2, DnaJ
计算分子量 241 aa, 27 kDa
观测分子量 27 kDa, 38 kDa
GenBank蛋白编号BC000177
基因名称 DNAJB6
Gene ID (NCBI) 10049
RRIDAB_3084261
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO75190
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJB6, belongs to the heat shock protein 40 (HSP40) family, a noncanonical member of the DNAJ-chaperone family, is mainly expressed in neurons. It plays an important role in recovery from misfolded protein aggregates (PMID: 27276715). As seen by the western blot stained for DNAJB6, there are 2 splice forms of the protein resulting in a longer isoform (38 kDa) and a shorter one (27 kDa). DNAJB6 (27 kDa) isoform but not DNAJB6 (38 kDa) isoform is effective with suppressing cytoplasmic protein aggregation (PMID: 20159555). The antibody 66587-1-Ig recognizes both isoforms.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 DNAJB6 antibody CL488-66587Download protocol
Standard Protocols
Click here to view our Standard Protocols