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验证数据展示

  • FC experiment of Jurkat using CL594-66587

    FC experiment of Jurkat using CL594-66587

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human DNAJB6 (CL594-66587, Clone:1A1D2) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated DNAJB6 Monoclonal antibody

DNAJB6 Monoclonal Antibody for FC (Intra)
Cat No. CL594-66587

Print datasheet

CloneNo. 1A1D2

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

FC (Intra)

Synonyms

DJ4, DKFZp566D0824, DnaJ, DNAJB6, FLJ42837, Heat shock protein J2, HHDJ1, HSJ 2, HSJ2, MRJ, MSJ 1, MSJ1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594

Size: 
100 μL

¥2800/ 100 μL

浓度: 1000 μg/ml

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经过测试的应用

Positive FC (Intra) detected inJurkat cells
Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 66587-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66587 targets DNAJB6 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen DNAJB6 fusion protein Ag2258 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily B, member 6
Synonyms DJ4, DKFZp566D0824, DnaJ, DNAJB6, FLJ42837, Heat shock protein J2, HHDJ1, HSJ 2, HSJ2, MRJ, MSJ 1, MSJ1
Calculated Molecular Weight 241 aa, 27 kDa
Observed Molecular Weight 27 kDa, 38 kDa
GenBank Accession NumberBC000177
Gene Symbol DNAJB6
Gene ID (NCBI) 10049
RRIDAB_3084716
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75190
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJB6, belongs to the heat shock protein 40 (HSP40) family, a noncanonical member of the DNAJ-chaperone family, is mainly expressed in neurons. It plays an important role in recovery from misfolded protein aggregates (PMID: 27276715). As seen by the western blot stained for DNAJB6, there are 2 splice forms of the protein resulting in a longer isoform (38 kDa) and a shorter one (27 kDa). DNAJB6 (27 kDa) isoform but not DNAJB6 (38 kDa) isoform is effective with suppressing cytoplasmic protein aggregation (PMID: 20159555). The antibody 66587-1-Ig recognizes both isoforms.

实验方案

Product Specific Protocols
FC protocol for CL594 DNAJB6 antibody CL594-66587Download protocol
Standard Protocols
Click here to view our Standard Protocols