验证数据展示
经过测试的应用
Positive FC (Intra) detected in | Jurkat cells |
Positive FC detected in | Jurkat cells |
推荐稀释比
应用 | 推荐稀释比 |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66587 targets DNAJB6 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat, Pig samples.
经测试应用 | FC (Intra) Application Description |
经测试反应性 | Human, Mouse, Rat, Pig |
免疫原 | DNAJB6 fusion protein Ag2258 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | DnaJ (Hsp40) homolog, subfamily B, member 6 |
别名 | DJ4, DKFZp566D0824, DnaJ, DNAJB6, FLJ42837, Heat shock protein J2, HHDJ1, HSJ 2, HSJ2, MRJ, MSJ 1, MSJ1 |
计算分子量 | 241 aa, 27 kDa |
观测分子量 | 27 kDa, 38 kDa |
GenBank蛋白编号 | BC000177 |
基因名称 | DNAJB6 |
Gene ID (NCBI) | 10049 |
RRID | AB_3084716 |
偶联类型 | CoraLite®594 Fluorescent Dye |
最大激发/发射波长 | 588 nm / 604 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | O75190 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNAJB6, belongs to the heat shock protein 40 (HSP40) family, a noncanonical member of the DNAJ-chaperone family, is mainly expressed in neurons. It plays an important role in recovery from misfolded protein aggregates (PMID: 27276715). As seen by the western blot stained for DNAJB6, there are 2 splice forms of the protein resulting in a longer isoform (38 kDa) and a shorter one (27 kDa). DNAJB6 (27 kDa) isoform but not DNAJB6 (38 kDa) isoform is effective with suppressing cytoplasmic protein aggregation (PMID: 20159555). The antibody 66587-1-Ig recognizes both isoforms.
实验方案
Product Specific Protocols | |
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FC protocol for CL594 DNAJB6 antibody CL594-66587 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |