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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DNAJC18 Polyclonal antibody

DNAJC18 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-25162

Print datasheet

Synonyms

DNAJC18

验证数据展示

  • FC experiment of U2OS using CL488-25162

    FC experiment of U2OS using CL488-25162

    1x10^6 U2OS cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Dnajc18 Polyclonal Antibody (CL488-25162)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inU2OS cells
Positive FC detected inU2OS cells
For other applications, we recommend the unconjugated version of this antibody, 25162-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-25162 targets DNAJC18 in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen DNAJC18 fusion protein Ag18498 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily C, member 18
Synonyms DNAJC18
Calculated Molecular Weight 358 aa, 42 kDa
Observed Molecular Weight 39-42 kDa
GenBank Accession NumberBC030162
Gene Symbol DNAJC18
Gene ID (NCBI) 202052
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9H819
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DnaJC18, also named as DnaJ (Hsp40) homolog, subfamily C, member 21, belongs to Type III DnaJ Family. Type III DnaJ proteins have a J domain but lack the other sequence features that are found in type I and II members of the family. DnaJC18 might play a role in the process of spermatogenesis in adult testis (PMID: 29082339).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 DNAJC18 antibody CL488-25162Download protocol
Standard Protocols
Click here to view our Standard Protocols