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Anti-Human DPP4/CD26 Rabbit Recombinant Antibody

DPP4/CD26 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

242045C1

Cat no : 98261-1-RR

Print datasheet

Synonyms

CD26, ADCP-2, ADCP2, ADCP 2, ADABP

验证数据展示

  • FC experiment of human PBMCs using 98261-1-RR

    FC experiment of human PBMCs using 98261-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human DPP4/CD26 Rabbit Recombinant Antibody (98261-1-RR, Clone: 242045C1) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 98261-1-RR

    FC experiment of human PBMCs using 98261-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human DPP4/CD26 Rabbit Recombinant Antibody (98261-1-RR, Clone: 242045C1) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

  • Affinity and Kinetic Characterization of 98261-1-RR

    Affinity and Kinetic Characterization of 98261-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98261-1-RR against Human DPP4/CD26 were performed. The affinity constant is 2.22 nM.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98261-1-RR targets DPP4/CD26 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name dipeptidyl-peptidase 4
Synonyms CD26, ADCP-2, ADCP2, ADCP 2, ADABP
Calculated Molecular Weight 88 kDa
GenBank Accession NumberBC013329
Gene Symbol DPP4
Gene ID (NCBI) 1803
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP27487
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580).

实验方案

Product Specific Protocols
FC protocol for DPP4/CD26 antibody 98261-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols