验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 81561-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-81561 targets DRP1 (C-terminal) in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | DRP1 (C-terminal) fusion protein Ag3644 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | dynamin 1-like |
| 别名 | DRP1, Dnm1p/Vps1p-like protein, DNM1L,DLP1, DNM1L, DLP1 |
| 计算分子量 | 736 aa, 82 kDa |
| 观测分子量 | 70-80 kDa |
| GenBank蛋白编号 | BC024590 |
| 基因名称 | DRP1 |
| Gene ID (NCBI) | 10059 |
| RRID | AB_3084503 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O00429 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 DRP1 (C-terminal) antibody CL488-81561 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |