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验证数据展示

  • IF Staining of HeLa using CL488-26187

    IF Staining of HeLa using CL488-26187

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 DRP1 (N-terminal) antibody (CL488-26187) at dilution of 1:200.

  • FC experiment of HeLa using CL488-26187

    FC experiment of HeLa using CL488-26187

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated DRP1 (N-terminal) Polyclonal antibody (CL488-26187)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated DRP1 (N-terminal) Polyclonal antibody

DRP1 (N-terminal) Polyclonal Antibody for IF/ICC
Cat No. CL488-26187

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC

别名

DRP1, Dymple, Dnm1p/Vps1p-like protein, DNM1L,DLP1, DNM1L

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 26187-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-26187 targets DRP1 (N-terminal) in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 DRP1 (N-terminal) fusion protein Ag24249 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dynamin 1-like
别名 DRP1, Dymple, Dnm1p/Vps1p-like protein, DNM1L,DLP1, DNM1L
计算分子量 736 aa, 82 kDa
观测分子量 70-78 kDa
GenBank蛋白编号BC024590
基因名称 DRP1
Gene ID (NCBI) 10059
RRIDAB_2919207
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00429
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DRP1 (N-terminal) antibody CL488-26187Download protocol
FC protocol for CL Plus 488 DRP1 (N-terminal) antibody CL488-26187Download protocol
Standard Protocols
Click here to view our Standard Protocols