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CoraLite® Plus 488-conjugated DSE Monoclonal antibody

DSE Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C9F7

Cat no : CL488-60041

Print datasheet

Synonyms

EC:5.1.3.19, DSEPI, DS epimerase, Dermatan-sulfate epimerase, Chondroitin-glucuronate 5-epimerase

验证数据展示

  • IF Staining of HeLa using CL488-60041

    IF Staining of HeLa using CL488-60041

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 DSE antibody (CL488-60041, Clone: 2C9F7 ) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-60041

    FC experiment of HeLa using CL488-60041

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated DSE Monoclonal antibody (CL488-60041, Clone:2C9F7)(red), or 0.4 ug Isotype Control (CL488-66360-1) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy DSE Ready-To-Use IHC Kit. DSE primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60041-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60041 targets DSE in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen DSE fusion protein Ag0695 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name dermatan sulfate epimerase
Synonyms EC:5.1.3.19, DSEPI, DS epimerase, Dermatan-sulfate epimerase, Chondroitin-glucuronate 5-epimerase
Calculated Molecular Weight 958 aa, 110 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC039245
Gene Symbol DSE
Gene ID (NCBI) 29940
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodEuglobulin precipitation
UNIPROT IDQ9UL01
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DSE, also named as SART2 and DSEPI, is an enzyme that converts D-glucuronic acid to L-iduronic acid residues in dermatan sulphate biosynthesis. It is also identified to be a tumour-associated antigen. DSE is recognized by cytotoxic T cells (CTLs) and its enhanced expression in many cancers has been reported. DSE is a potential candidate for a tumour antigen with immunogenicity.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DSE antibody CL488-60041Download protocol
FC protocol for CL Plus 488 DSE antibody CL488-60041Download protocol
Standard Protocols
Click here to view our Standard Protocols