• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HeLa using CL488-60041

    IF Staining of HeLa using CL488-60041

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 DSE antibody (CL488-60041, Clone: 2C9F7 ) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-60041

    FC experiment of HeLa using CL488-60041

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated DSE Monoclonal antibody (CL488-60041, Clone:2C9F7)(red), or 0.4 ug Isotype Control (CL488-66360-1) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated DSE Monoclonal antibody

DSE Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-60041

产品说明书

CloneNo. 2C9F7

宿主/亚型

Mouse / IgM

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

EC:5.1.3.19, DSEPI, DS epimerase, Dermatan-sulfate epimerase, Chondroitin-glucuronate 5-epimerase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy DSE Ready-To-Use IHC Kit. DSE primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60041-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60041 targets DSE in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 DSE fusion protein Ag0695 种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 dermatan sulfate epimerase
别名 EC:5.1.3.19, DSEPI, DS epimerase, Dermatan-sulfate epimerase, Chondroitin-glucuronate 5-epimerase
计算分子量 958 aa, 110 kDa
观测分子量 100 kDa
GenBank蛋白编号BC039245
基因名称 DSE
Gene ID (NCBI) 29940
RRIDAB_3672860
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Euglobulin precipitation
UNIPROT IDQ9UL01
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DSE, also named as SART2 and DSEPI, is an enzyme that converts D-glucuronic acid to L-iduronic acid residues in dermatan sulphate biosynthesis. It is also identified to be a tumour-associated antigen. DSE is recognized by cytotoxic T cells (CTLs) and its enhanced expression in many cancers has been reported. DSE is a potential candidate for a tumour antigen with immunogenicity.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DSE antibody CL488-60041Download protocol
FC protocol for CL Plus 488 DSE antibody CL488-60041Download protocol
Standard Protocols
Click here to view our Standard Protocols