验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | PC-12 cells, HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83355-3-RR targets DUSP6 in FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Applications | FC (Intra), ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | DUSP6 fusion protein Ag0705 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | dual specificity phosphatase 6 |
| Synonyms | MKP 3, Mitogen-activated protein kinase phosphatase 3, MAP kinase phosphatase 3, EC:3.1.3.16, dual specificity phosphatase 6 |
| Calculated Molecular Weight | 42 kDa |
| GenBank Accession Number | BC003562 |
| Gene Symbol | DUSP6 |
| Gene ID (NCBI) | 1848 |
| RRID | AB_3671012 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q16828 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Dual specificity phosphatase 6 (DUSP6) is a member of the family of mitogen-activated protein kinase (MAPK) phosphatases, which dephosphorylate and inactivate MAPK (PMID: 9858808). The MAPK pathway is an important signal transduction system that serves cellular processes, such as proliferation, differentiation, migration, and apoptosis. DUSP6 resides at chromosome location 12q22-23, which is one of the candidate loci for susceptibility to bipolar disorder and which encodes a phosphatase selective for extracellular signal-regulated kinase (ERK) (PMID: 9205128). DUSP6 inactivates extracellular signal-regulated kinase (ERK), and can act in tumor suppressive pathways, whose expression is reduced in several different types of cancer. (PMID: 21680106).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for DUSP6 antibody 83355-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |