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验证数据展示

  • WB analysis using 67672-1-Ig

    WB analysis using 67672-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67672-1-Ig (DVL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat colon using 67672-1-Ig

    IHC staining of rat colon using 67672-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67672-1-Ig (DVL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat colon using 67672-1-Ig

    IHC staining of rat colon using 67672-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67672-1-Ig (DVL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DVL1 Monoclonal antibody

DVL1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67672-1-Ig
CloneNo. 1A2A5

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IHC, ELISA

Synonyms

Dishevelled 1, DSH homolog 1, DVL, DVL1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, ROS1728 cells, NCCIT cells, HeLa cells, MDA-MB-231 cells
Positive IHC detected inrat colon tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67672-1-Ig targets DVL1 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, rat, mouse
Immunogen DVL1 fusion protein Ag26083 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name dishevelled, dsh homolog 1 (Drosophila)
Synonyms Dishevelled 1, DSH homolog 1, DVL, DVL1
Calculated Molecular Weight 75 kDa
Observed Molecular Weight70-75 kDa
GenBank Accession NumberBC050454
Gene Symbol DVL1
Gene ID (NCBI) 1855
RRIDAB_2882868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO14640
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

DVL1 is one of three DVL homologous proteins (DVL1-3) widely expressed in embryonic development and in the adult central nervous system. Dishevelled proteins are a necessary component of the Wnt and planar cell polarity developmental signaling pathways. Overexpression of DVL1 has been linked to prostate and breast cancers through the Wnt signaling pathway. There are two isoforms of DVL1: 75 kDa and 73 kDa.

实验方案

Product Specific Protocols
WB protocol for DVL1 antibody 67672-1-IgDownload protocol
IHC protocol for DVL1 antibody 67672-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols