验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, ROS1728 cells, NCCIT cells, HeLa cells, MDA-MB-231 cells |
Positive IHC detected in | rat colon tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67672-1-Ig targets DVL1 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | Human, rat, mouse |
Immunogen | DVL1 fusion protein Ag26083 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | dishevelled, dsh homolog 1 (Drosophila) |
Synonyms | Dishevelled 1, DSH homolog 1, DVL, DVL1 |
Calculated Molecular Weight | 75 kDa |
Observed Molecular Weight | 70-75 kDa |
GenBank Accession Number | BC050454 |
Gene Symbol | DVL1 |
Gene ID (NCBI) | 1855 |
RRID | AB_2882868 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | O14640 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DVL1 is one of three DVL homologous proteins (DVL1-3) widely expressed in embryonic development and in the adult central nervous system. Dishevelled proteins are a necessary component of the Wnt and planar cell polarity developmental signaling pathways. Overexpression of DVL1 has been linked to prostate and breast cancers through the Wnt signaling pathway. There are two isoforms of DVL1: 75 kDa and 73 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for DVL1 antibody 67672-1-Ig | Download protocol |
IHC protocol for DVL1 antibody 67672-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |