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验证数据展示

  • IF Staining of MCF-7 using CL488-67672

    IF Staining of MCF-7 using CL488-67672

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 DVL1 antibody (CL488-67672, Clone: 1A2A5 ) at dilution of 1:200.

  • FC experiment of U2OS using CL488-67672

    FC experiment of U2OS using CL488-67672

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated DVL1 Monoclonal antibody (CL488-67672, Clone:1A2A5)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated DVL1 Monoclonal antibody

DVL1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67672

产品说明书

CloneNo. 1A2A5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

Segment polarity protein dishevelled homolog DVL-1, DVL, DSH homolog 1, Dishevelled-1, Dishevelled 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inU2OS cells
For other applications, we recommend the unconjugated version of this antibody, 67672-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67672 targets DVL1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 DVL1 fusion protein Ag26083 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 dishevelled, dsh homolog 1 (Drosophila)
别名 Segment polarity protein dishevelled homolog DVL-1, DVL, DSH homolog 1, Dishevelled-1, Dishevelled 1
计算分子量 75 kDa
观测分子量70-75 kDa
GenBank蛋白编号BC050454
基因名称 DVL1
Gene ID (NCBI) 1855
RRIDAB_2934559
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO14640
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DVL1 is one of three DVL homologous proteins (DVL1-3) widely expressed in embryonic development and in the adult central nervous system. Dishevelled proteins are a necessary component of the Wnt and planar cell polarity developmental signaling pathways. Overexpression of DVL1 has been linked to prostate and breast cancers through the Wnt signaling pathway. There are two isoforms of DVL1: 75 kDa and 73 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DVL1 antibody CL488-67672Download protocol
Standard Protocols
Click here to view our Standard Protocols