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CoraLite® Plus 488-conjugated DVL1 Monoclonal antibody

DVL1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A2A5

Cat no : CL488-67672

Print datasheet

Synonyms

Segment polarity protein dishevelled homolog DVL-1, DVL, DSH homolog 1, Dishevelled-1, Dishevelled 1

验证数据展示

  • IF Staining of MCF-7 using CL488-67672

    IF Staining of MCF-7 using CL488-67672

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 DVL1 antibody (CL488-67672, Clone: 1A2A5 ) at dilution of 1:200.

  • FC experiment of U2OS using CL488-67672

    FC experiment of U2OS using CL488-67672

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated DVL1 Monoclonal antibody (CL488-67672, Clone:1A2A5)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inU2OS cells
For other applications, we recommend the unconjugated version of this antibody, 67672-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67672 targets DVL1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen DVL1 fusion protein Ag26083 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name dishevelled, dsh homolog 1 (Drosophila)
Synonyms Segment polarity protein dishevelled homolog DVL-1, DVL, DSH homolog 1, Dishevelled-1, Dishevelled 1
Calculated Molecular Weight 75 kDa
Observed Molecular Weight70-75 kDa
GenBank Accession NumberBC050454
Gene Symbol DVL1
Gene ID (NCBI) 1855
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO14640
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DVL1 is one of three DVL homologous proteins (DVL1-3) widely expressed in embryonic development and in the adult central nervous system. Dishevelled proteins are a necessary component of the Wnt and planar cell polarity developmental signaling pathways. Overexpression of DVL1 has been linked to prostate and breast cancers through the Wnt signaling pathway. There are two isoforms of DVL1: 75 kDa and 73 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DVL1 antibody CL488-67672Download protocol
Standard Protocols
Click here to view our Standard Protocols