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Di-Methyl-Histone H3 (Arg2) Recombinant antibody, PBS Only

Di-Methyl-Histone H3 (Arg2) Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Dot Blot, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Dot Blot, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241896D7

Cat no : 84702-1-PBS

Print datasheet

Synonyms

HIST1H3A, 241896D7, Histone-H3, HistoneH3, Histone H3

验证数据展示

  • Dot Blot experiment of peptide using 84702-1-RR (same clone as 84702-1-PBS)

    Dot Blot experiment of peptide using 84702-1-RR (same clone as 84702-1-PBS)

    Dot blot analysis was used to confirm the specificity of 84702-1-RR Di-Methyl-Histone H3 (Arg2) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 84702-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84702-1-RR (same clone as 84702-1-PBS)

    IF Staining of HeLa using 84702-1-RR (same clone as 84702-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84702-1-RR, Clone: 241896D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84702-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84702-1-RR (same clone as 84702-1-PBS)

    FC experiment of HeLa using 84702-1-RR (same clone as 84702-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Hist1h3a Recombinant Antibody (84702-1-RR, Clone:241896D7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84702-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

产品信息

84702-1-PBS targets Di-Methyl-Histone H3 (Arg2) in IF/ICC, FC (Intra), Dot Blot, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), Dot Blot, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histone cluster 1, H3a
Synonyms HIST1H3A, 241896D7, Histone-H3, HistoneH3, Histone H3
GenBank Accession NumberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP68431
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C