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验证数据展示

  • WB analysis using 68120-1-Ig (same clone as 68120-1-PBS)

    WB analysis using 68120-1-Ig (same clone as 68120-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

  • IHC staining of rat skeletal muscle using 68120-1-Ig (same clone as 68120-1-PBS)

    IHC staining of rat skeletal muscle using 68120-1-Ig (same clone as 68120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68120-1-Ig (Dystrophin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Dystrophin/DMD antibody included
  • IF Staining of mouse skeletal muscle using 68120-1-Ig (same clone as 68120-1-PBS)

    IF Staining of mouse skeletal muscle using 68120-1-Ig (same clone as 68120-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Dystrophin antibody (68120-1-Ig, Clone: 1G12E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 68120-1-Ig (same clone as 68120-1-PBS)

    FC experiment of HepG2 using 68120-1-Ig (same clone as 68120-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Dystrophin (68120-1-Ig, Clone:1G12E6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 68120-1-Ig (same clone as 68120-1-PBS)

    WB analysis of HeLa using 68120-1-Ig (same clone as 68120-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

  • WB analysis of rat heart using 68120-1-Ig (same clone as 68120-1-PBS)

    WB analysis of rat heart using 68120-1-Ig (same clone as 68120-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

  • WB analysis using 68120-1-Ig (same clone as 68120-1-PBS)

    WB analysis using 68120-1-Ig (same clone as 68120-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68120-1-Ig (DMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 68120-1-Ig (same clone as 68120-1-PBS)

    IHC staining of mouse skeletal muscle using 68120-1-Ig (same clone as 68120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68120-1-Ig (Dystrophin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Dystrophin/DMD antibody included
  • IHC staining of mouse skeletal muscle using 68120-1-Ig (same clone as 68120-1-PBS)

    IHC staining of mouse skeletal muscle using 68120-1-Ig (same clone as 68120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68120-1-Ig (Dystrophin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Dystrophin/DMD antibody included
  • IF Staining of rat heart using 68120-1-Ig (same clone as 68120-1-PBS)

    IF Staining of rat heart using 68120-1-Ig (same clone as 68120-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using Dystrophin antibody (68120-1-Ig, Clone: 1G12E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

Dystrophin Monoclonal antibody, PBS Only

Dystrophin Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 68120-1-PBS

产品说明书

CloneNo. 1G12E6

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, FC (Intra), Indirect ELISA

别名

DMD, BMD, 1G12E6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Dystrophin/DMD Ready-To-Use IHC Kit. Dystrophin/DMD primary antibody included.

产品信息

68120-1-PBS targets Dystrophin in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Dystrophin fusion protein Ag4392 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 dystrophin
别名 DMD, BMD, 1G12E6
计算分子量 3685 aa, 427 kDa
观测分子量70 kDa, 430 kDa
GenBank蛋白编号BC028720
基因名称 Dystrophin
Gene ID (NCBI) 1756
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP11532
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW (69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.