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Dystrophin Monoclonal antibody, PBS Only

Dystrophin Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G12E6

Cat no : 68120-1-PBS

Print datasheet

Synonyms

DMD, BMD, 1G12E6

验证数据展示

  • WB analysis using 68120-1-Ig (same clone as 68120-1-PBS)

    WB analysis using 68120-1-Ig (same clone as 68120-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

  • IHC staining of rat skeletal muscle using 68120-1-Ig (same clone as 68120-1-PBS)

    IHC staining of rat skeletal muscle using 68120-1-Ig (same clone as 68120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68120-1-Ig (Dystrophin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Dystrophin/DMD antibody included
  • IF Staining of mouse skeletal muscle using 68120-1-Ig (same clone as 68120-1-PBS)

    IF Staining of mouse skeletal muscle using 68120-1-Ig (same clone as 68120-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Dystrophin antibody (68120-1-Ig, Clone: 1G12E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 68120-1-Ig (same clone as 68120-1-PBS)

    FC experiment of HepG2 using 68120-1-Ig (same clone as 68120-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Dystrophin (68120-1-Ig, Clone:1G12E6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

  • WB analysis using 68120-1-Ig (same clone as 68120-1-PBS)

    WB analysis using 68120-1-Ig (same clone as 68120-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68120-1-Ig (DMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 68120-1-Ig (same clone as 68120-1-PBS)

    WB analysis of HeLa using 68120-1-Ig (same clone as 68120-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

  • WB analysis of rat heart using 68120-1-Ig (same clone as 68120-1-PBS)

    WB analysis of rat heart using 68120-1-Ig (same clone as 68120-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 68120-1-Ig (same clone as 68120-1-PBS)

    IHC staining of mouse skeletal muscle using 68120-1-Ig (same clone as 68120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68120-1-Ig (Dystrophin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Dystrophin/DMD antibody included
  • IF Staining of rat heart using 68120-1-Ig (same clone as 68120-1-PBS)

    IF Staining of rat heart using 68120-1-Ig (same clone as 68120-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using Dystrophin antibody (68120-1-Ig, Clone: 1G12E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Dystrophin/DMD Ready-To-Use IHC Kit. Dystrophin/DMD primary antibody included.

产品信息

68120-1-PBS targets Dystrophin in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Dystrophin fusion protein Ag4392 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name dystrophin
Synonyms DMD, BMD, 1G12E6
Calculated Molecular Weight 3685 aa, 427 kDa
Observed Molecular Weight70 kDa, 430 kDa
GenBank Accession NumberBC028720
Gene Symbol Dystrophin
Gene ID (NCBI) 1756
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11532
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW (69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.