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Dystrophin Monoclonal antibody

Dystrophin Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1G12E6

Cat no : 68120-1-Ig

Print datasheet

Synonyms

DMD, BMD, 1G12E6

验证数据展示

  • WB analysis using 68120-1-Ig

    WB analysis using 68120-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat skeletal muscle using 68120-1-Ig

    IHC staining of rat skeletal muscle using 68120-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68120-1-Ig (Dystrophin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Dystrophin/DMD antibody included
  • IF Staining of mouse skeletal muscle using 68120-1-Ig

    IF Staining of mouse skeletal muscle using 68120-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Dystrophin antibody (68120-1-Ig, Clone: 1G12E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • FC experiment of HepG2 using 68120-1-Ig

    FC experiment of HepG2 using 68120-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Dystrophin (68120-1-Ig, Clone:1G12E6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 68120-1-Ig

    WB analysis using 68120-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68120-1-Ig (DMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 68120-1-Ig

    WB analysis of HeLa using 68120-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat heart using 68120-1-Ig

    WB analysis of rat heart using 68120-1-Ig

    rat heart tissue were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 68120-1-Ig

    IHC staining of mouse skeletal muscle using 68120-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68120-1-Ig (Dystrophin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Dystrophin/DMD antibody included
  • IF Staining of rat heart using 68120-1-Ig

    IF Staining of rat heart using 68120-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using Dystrophin antibody (68120-1-Ig, Clone: 1G12E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inrat brain tissue, HeLa cells, rat heart tissue, HepG2 cells, mouse brain tissue
Positive IHC detected inrat skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue, rat heart tissue
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy Dystrophin/DMD Ready-To-Use IHC Kit. Dystrophin/DMD primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68120-1-Ig targets Dystrophin in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Dystrophin fusion protein Ag4392 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name dystrophin
Synonyms DMD, BMD, 1G12E6
Calculated Molecular Weight 3685 aa, 427 kDa
Observed Molecular Weight70 kDa, 430 kDa
GenBank Accession NumberBC028720
Gene Symbol Dystrophin
Gene ID (NCBI) 1756
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11532
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW (69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.

实验方案

Product Specific Protocols
WB protocol for Dystrophin antibody 68120-1-IgDownload protocol
IHC protocol for Dystrophin antibody 68120-1-IgDownload protocol
IF protocol for Dystrophin antibody 68120-1-IgDownload protocol
FC protocol for Dystrophin antibody 68120-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols