CoraLite® Plus 488-conjugated Dystrophin Monoclonal antibody
Dystrophin Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
IF/ICC, IF-P, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1G12E6
验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse skeletal muscle tissue |
| Positive IF/ICC detected in | H9C2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy Dystrophin/DMD Ready-To-Use IHC Kit. Dystrophin/DMD primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68120-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68120 targets Dystrophin in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IF-P, FC (Intra) Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | Dystrophin fusion protein Ag4392 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | dystrophin |
| Synonyms | DMD, BMD, 1G12E6 |
| Calculated Molecular Weight | 3685 aa, 427 kDa |
| Observed Molecular Weight | 70 kDa, 430 kDa |
| GenBank Accession Number | BC028720 |
| Gene Symbol | Dystrophin |
| Gene ID (NCBI) | 1756 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P11532 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW (69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Dystrophin antibody CL488-68120 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
