验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse skeletal muscle tissue |
| Positive IF/ICC detected in | H9C2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68120 targets Dystrophin in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC, IF-P, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | Dystrophin fusion protein Ag4392 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | dystrophin |
| 别名 | DMD, BMD, 1G12E6 |
| 计算分子量 | 3685 aa, 427 kDa |
| 观测分子量 | 70 kDa, 430 kDa |
| GenBank蛋白编号 | BC028720 |
| 基因名称 | Dystrophin |
| Gene ID (NCBI) | 1756 |
| RRID | AB_3084404 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P11532 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW (69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Dystrophin antibody CL488-68120 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |