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  • Featured Product
  • KD/KO Validated

E-cadherin Monoclonal antibody, PBS Only

E-cadherin Monoclonal Antibody for WB,Indirect ELISA,IHC,IF,FC

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig, rat

Applications

WB,Indirect ELISA,IHC,IF,FC

Conjugate

Unconjugated

CloneNo.

6B11F11

Cat No : 60335-1-PBS

Print datasheet

Synonyms

Arc 1, Cadherin 1, CAM 120/80, CD324, CDH1, CDHE, E cadherin, ECAD, E-cadherin, Epithelial cadherin, LCAM, UVO, Uvomorulin

验证数据展示

  • WB analysis of A431 using 60335-1-Ig (same clone as 60335-1-PBS)

    WB analysis of A431 using 60335-1-Ig (same clone as 60335-1-PBS)

    WB result of E-cadherin antibody (60335-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E-cadherin transfected A431 cells. This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • WB analysis of PC-3 using 60335-1-Ig (same clone as 60335-1-PBS)

    WB analysis of PC-3 using 60335-1-Ig (same clone as 60335-1-PBS)

    PC-3, MKN-45, SGC-7901 cells were subjected to SDS PAGE followed by western blot with 60335-1-Ig (E-cadherin Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • IF Staining of human kidney using 60335-1-Ig (same clone as 60335-1-PBS)

    IF Staining of human kidney using 60335-1-Ig (same clone as 60335-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:300 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), (18150-1-AP, green). DNA was stained by DAPI (blue). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 60335-1-Ig (same clone as 60335-1-PBS)

    IF Staining of human breast cancer using 60335-1-Ig (same clone as 60335-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60335-1-Ig (same clone as 60335-1-PBS)

    IHC staining of human breast cancer using 60335-1-Ig (same clone as 60335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60335-1-Ig (same clone as 60335-1-PBS)

    IHC staining of human breast cancer using 60335-1-Ig (same clone as 60335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 60335-1-Ig (same clone as 60335-1-PBS)

    FC experiment of A431 using 60335-1-Ig (same clone as 60335-1-PBS)

    1X10^6 A431 cells were stained with 0.2 ug Anti-Human E-cadherin (60335-1-Ig, Clone:6B11F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug isotype control antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 60335-1-Ig (same clone as 60335-1-PBS)

    IF Staining of MCF-7 using 60335-1-Ig (same clone as 60335-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

产品信息

60335-1-PBS targets E-cadherin in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, pig, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF,FC
Tested Reactivity human, pig, rat
Immunogen E-cadherin fusion protein Ag15085 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Synonyms Arc 1, Cadherin 1, CAM 120/80, CD324, CDH1, CDHE, E cadherin, ECAD, E-cadherin, Epithelial cadherin, LCAM, UVO, Uvomorulin
Calculated Molecular Weight 882 aa, 97 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC141838
Gene Symbol CDH1
Gene ID (NCBI) 999
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12830
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.