验证数据展示

WB analysis of A431 using 60335-1-Ig
WB result of E-cadherin antibody (60335-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E-cadherin transfected A431 cells.
WB analysis of PC-3 using 60335-1-Ig
PC-3, MKN-45, SGC-7901 cells were subjected to SDS PAGE followed by western blot with 60335-1-Ig (E-cadherin Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IF Staining of human kidney using 60335-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:300 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), (18150-1-AP, green). DNA was stained by DAPI (blue).
IF Staining of human breast cancer using 60335-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of MCF-7 using 60335-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IHC staining of human breast cancer using 60335-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with E-cadherin antibody included
IHC staining of human breast cancer using 60335-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with E-cadherin antibody included
IHC staining of human breast cancer using 60335-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:8000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with E-cadherin antibody included
IF Staining of mouse brain using 60335-1-Ig
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).
IF Staining of mouse breast cancer using 60335-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse breast cancer using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

Featured Product
KD/KO验证

E-cadherin Monoclonal antibody

E-cadherin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Cat No. 60335-1-Ig

产品说明书

CloneNo. 6B11F11

引用文献(229)

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig and More (1)

应用

WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA and More (1)

别名

6B11F11, Arc 1, Cadherin 1, Cadherin-1, CAM 120/80

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®555 CoraLite®594

规格	价格	库存

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点击咨询售后客服

概述

经过测试的应用
产品信息
发表文章 (229)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	PC-3 cells, A431 cells, MCF-7 cells, pig brain tissue, MKN-45 cells, SGC-7901 cells
Positive IHC detected in	human breast cancer tissue, human colon tissue, rat colon tissue, rat stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human breast cancer tissue, human kidney tissue, MCF-7 cells
Positive IF-Fro detected in	mouse brain tissue
Positive IF/ICC detected in	MCF-7 cells, mouse breast cancer

Planning an IHC experiment? We recommend our IHCeasy E-cadherin Ready-To-Use IHC Kit. E-cadherin primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:8000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Immunofluorescence (IF)-FRO	IF-FRO : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 192 publications below
IHC	See 28 publications below
IF	See 57 publications below

产品信息

60335-1-Ig targets E-cadherin in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用	WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse, rat, pig
文献引用反应性	human, mouse, rat, pig, monkey
免疫原	CatNo: Ag15085 Product name: Recombinant human E-cadherin protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 373-622 aa of BC141838 Sequence: PIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPI 种属同源性预测
宿主/亚型	Mouse / IgG2b
抗体类别	Monoclonal
产品类型	Antibody
全称	cadherin 1, type 1, E-cadherin (epithelial)
别名	6B11F11, Arc 1, Cadherin 1, Cadherin-1, CAM 120/80
计算分子量	882 aa, 97 kDa
观测分子量	120 kDa
GenBank蛋白编号	BC141838
基因名称	E-cadherin
Gene ID (NCBI)	999
RRID	AB_2881444
偶联类型	Unconjugated
形式	Liquid
纯化方式	Protein A purification
UNIPROT ID	P12830
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

实验方案

Product Specific Protocols
IF protocol for E-cadherin antibody 60335-1-Ig	Download protocol
IHC protocol for E-cadherin antibody 60335-1-Ig	Download protocol
WB protocol for E-cadherin antibody 60335-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	IHC	Adv Mater Glycated ECM Derived Carbon Dots Inhibit Tumor Vasculogenic Mimicry by Disrupting RAGE Nuclear Translocation and Its Interaction With HMGB1 Authors - Shaoting Zheng View Article
human	IF	Theranostics FSH induces EMT in ovarian cancer via ALKBH5-regulated Snail m6A demethylation Authors - Xingyan Xu View Article
mouse	WB,IHC	Theranostics Vitamin D binding protein (VDBP) hijacks twist1 to inhibit vasculogenic mimicry in hepatocellular carcinoma Authors - Lu-Ning Qin View Article
human	WB,IHC	EBioMedicine PMID: 31594754 Authors - Yiyi Liu View Article
	IHC	J Adv Res HnRNPR-mediated UPF3B mRNA splicing drives hepatocellular carcinoma metastasis Authors - Hong Wang View Article
human	WB	Biomaterials Urinary exosomes-based Engineered Nanovectors for Homologously Targeted Chemo-Chemodynamic Prostate Cancer Therapy via abrogating IGFR/AKT/NF-kB/IkB signaling. Authors - Shaojun Pan View Article

验证数据展示

WB analysis of A431 using 60335-1-Ig

WB analysis of PC-3 using 60335-1-Ig

IF Staining of human kidney using 60335-1-Ig

IF Staining of human breast cancer using 60335-1-Ig

IF Staining of MCF-7 using 60335-1-Ig

IHC staining of human breast cancer using 60335-1-Ig

IHC staining of human breast cancer using 60335-1-Ig

IHC staining of human breast cancer using 60335-1-Ig

IF Staining of mouse brain using 60335-1-Ig

IF Staining of mouse breast cancer using 60335-1-Ig

E-cadherin Monoclonal antibody

E-cadherin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Glycated ECM Derived Carbon Dots Inhibit Tumor Vasculogenic Mimicry by Disrupting RAGE Nuclear Translocation and Its Interaction With HMGB1

FSH induces EMT in ovarian cancer via ALKBH5-regulated Snail m6A demethylation

Vitamin D binding protein (VDBP) hijacks twist1 to inhibit vasculogenic mimicry in hepatocellular carcinoma

PMID: 31594754

HnRNPR-mediated UPF3B mRNA splicing drives hepatocellular carcinoma metastasis

Urinary exosomes-based Engineered Nanovectors for Homologously Targeted Chemo-Chemodynamic Prostate Cancer Therapy via abrogating IGFR/AKT/NF-kB/IkB signaling.