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CoraLite® Plus 488-conjugated E-cadherin Monoclonal antibody

E-cadherin Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL488-60335

产品说明书

CloneNo. 6B11F11

宿主/亚型

Mouse / IgG2b

种属反应性

human, pig

应用

IF/ICC, IF-P

CD324, CAM 120/80, Cadherin-1, Cadherin 1, Arc 1

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive IF-P detected inhuman breast cancer tissue
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy E-cadherin Ready-To-Use IHC Kit. E-cadherin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60335-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60335 targets E-cadherin in IF/ICC, IF-P applications and shows reactivity with human, pig samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human, pig
免疫原 E-cadherin fusion protein Ag15085 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cadherin 1, type 1, E-cadherin (epithelial)
别名 CD324, CAM 120/80, Cadherin-1, Cadherin 1, Arc 1
计算分子量 882 aa, 97 kDa
观测分子量 120 kDa
GenBank蛋白编号BC141838
基因名称 E-cadherin
Gene ID (NCBI) 999
RRIDAB_2919249
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP12830
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 E-cadherin antibody CL488-60335Download protocol
Standard Protocols
Click here to view our Standard Protocols
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