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CoraLite®594-conjugated E-cadherin Monoclonal antibody

E-cadherin Monoclonal Antibody for IF-P
Cat No. CL594-60335

产品说明书

CloneNo. 6B11F11

宿主/亚型

Mouse / IgG2b

种属反应性

human, pig

应用

IF-P

CD324, CAM 120/80, Cadherin-1, Cadherin 1, Arc 1

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive IF-P detected inhuman breast cancer tissue
Planning an IHC experiment? We recommend our IHCeasy E-cadherin Ready-To-Use IHC Kit. E-cadherin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60335-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60335 targets E-cadherin in IF-P applications and shows reactivity with human, pig samples.

经测试应用 IF-P Application Description
经测试反应性 human, pig
免疫原 E-cadherin fusion protein Ag15085 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cadherin 1, type 1, E-cadherin (epithelial)
别名 CD324, CAM 120/80, Cadherin-1, Cadherin 1, Arc 1
计算分子量 882 aa, 97 kDa
观测分子量 120 kDa
GenBank蛋白编号BC141838
基因名称 E-cadherin
Gene ID (NCBI) 999
RRIDAB_2919918
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP12830
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

实验方案

Product Specific Protocols
IF protocol for CL594 E-cadherin antibody CL594-60335Download protocol
Standard Protocols
Click here to view our Standard Protocols
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