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E-cadherin Monoclonal antibody
E-cadherin Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, pig, rat and More (1)
Applications
FC, IF, IHC, WB, ELISA
Conjugate
Unconjugated
154
CloneNo.
6B11F11
验证数据展示
经过测试的应用
Positive WB detected in | PC-3 cells, A431 cells, MCF-7 cells, pig brain tissue, MKN-45 cells, SGC-7901 cells |
Positive IHC detected in | human breast cancer tissue, rat stomach tissue, human colon tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human breast cancer tissue, human kidney tissue, MCF-7 cells |
Positive FC detected in | A431 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
60335-1-Ig targets E-cadherin in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, pig, rat samples.
Tested Applications | FC, IF, IHC, WB, ELISA |
Cited Applications | FC, IF, IHC, WB |
Tested Reactivity | human, pig, rat |
Cited Reactivity | human, rat, monkey, pig |
Immunogen | E-cadherin fusion protein Ag15085 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | cadherin 1, type 1, E-cadherin (epithelial) |
Synonyms | Arc 1, Cadherin 1, CAM 120/80, CD324, CDH1, CDHE, E cadherin, ECAD, E-cadherin, Epithelial cadherin, LCAM, UVO, Uvomorulin |
Calculated Molecular Weight | 882 aa, 97 kDa |
Observed Molecular Weight | 120 kDa |
GenBank Accession Number | BC141838 |
Gene Symbol | CDH1 |
Gene ID (NCBI) | 999 |
RRID | AB_2881444 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P12830 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for E-cadherin antibody 60335-1-Ig | Download protocol |
IHC protocol for E-cadherin antibody 60335-1-Ig | Download protocol |
IF protocol for E-cadherin antibody 60335-1-Ig | Download protocol |
FC protocol for E-cadherin antibody 60335-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Biomaterials Urinary exosomes-based Engineered Nanovectors for Homologously Targeted Chemo-Chemodynamic Prostate Cancer Therapy via abrogating IGFR/AKT/NF-kB/IkB signaling. | ||
Redox Biol Riboflavin deficiency leads to irreversible cellular changes in the RPE and disrupts retinal function through alterations in cellular metabolic homeostasis. | ||
Acta Pharmacol Sin GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy | ||
Mucosal Immunol Airway epithelium IgE-FcεRI Cross-Link Induces Epithelial Barrier Disruption in Severe T2-high Asthma |