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E-cadherin Monoclonal antibody

E-cadherin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA
Cat No. 60335-1-Ig

产品说明书

CloneNo. 6B11F11

宿主/亚型

Mouse / IgG2b

种属反应性

human, rat, pig and More (1)

应用

WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA and More (1)

CD324, CAM 120/80, Cadherin-1, Cadherin 1, Arc 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inPC-3 cells, A431 cells, MCF-7 cells, pig brain tissue, MKN-45 cells, SGC-7901 cells
Positive IHC detected inhuman breast cancer tissue, human colon tissue, rat colon tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue, human kidney tissue, MCF-7 cells
Positive IF-Fro detected inmouse brain tissue
Positive IF/ICC detected inMCF-7 cells, mouse breast cancer
Planning an IHC experiment? We recommend our IHCeasy E-cadherin Ready-To-Use IHC Kit. E-cadherin primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)-FROIF-FRO : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60335-1-Ig targets E-cadherin in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, rat, pig
文献引用反应性human, rat, pig, monkey
免疫原 E-cadherin fusion protein Ag15085 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cadherin 1, type 1, E-cadherin (epithelial)
别名 CD324, CAM 120/80, Cadherin-1, Cadherin 1, Arc 1
计算分子量 882 aa, 97 kDa
观测分子量 120 kDa
GenBank蛋白编号BC141838
基因名称 E-cadherin
Gene ID (NCBI) 999
RRIDAB_2881444
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP12830
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

实验方案

Product Specific Protocols
WB protocol for E-cadherin antibody 60335-1-IgDownload protocol
IHC protocol for E-cadherin antibody 60335-1-IgDownload protocol
IF protocol for E-cadherin antibody 60335-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB,IHC

Theranostics

Vitamin D binding protein (VDBP) hijacks twist1 to inhibit vasculogenic mimicry in hepatocellular carcinoma

Authors - Lu-Ning Qin
humanIF

Theranostics

FSH induces EMT in ovarian cancer via ALKBH5-regulated Snail m6A demethylation

Authors - Xingyan Xu
humanWB,IHC

EBioMedicine

PMID: 31594754

Authors - Yiyi Liu
IHC

J Adv Res

HnRNPR-mediated UPF3B mRNA splicing drives hepatocellular carcinoma metastasis

Authors - Hong Wang
humanWB

Biomaterials

Urinary exosomes-based Engineered Nanovectors for Homologously Targeted Chemo-Chemodynamic Prostate Cancer Therapy via abrogating IGFR/AKT/NF-kB/IkB signaling.

Authors - Shaojun Pan
WB

Redox Biol

Riboflavin deficiency leads to irreversible cellular changes in the RPE and disrupts retinal function through alterations in cellular metabolic homeostasis.

Authors - Tirthankar Sinha
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