验证数据展示
经过测试的应用
Positive WB detected in | PC-3 cells, A431 cells, MCF-7 cells, pig brain tissue, MKN-45 cells, SGC-7901 cells |
Positive IHC detected in | human breast cancer tissue, human colon tissue, rat colon tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human breast cancer tissue, human kidney tissue, MCF-7 cells |
Positive IF-Fro detected in | mouse brain tissue |
Positive IF/ICC detected in | MCF-7 cells, mouse breast cancer |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
60335-1-Ig targets E-cadherin in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, rat, pig samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, rat, pig |
Cited Reactivity | human, rat, pig, monkey |
Immunogen | E-cadherin fusion protein Ag15085 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | cadherin 1, type 1, E-cadherin (epithelial) |
Synonyms | CD324, CAM 120/80, Cadherin-1, Cadherin 1, Arc 1 |
Calculated Molecular Weight | 882 aa, 97 kDa |
Observed Molecular Weight | 120 kDa |
GenBank Accession Number | BC141838 |
Gene Symbol | E-cadherin |
Gene ID (NCBI) | 999 |
RRID | AB_2881444 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P12830 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.
实验方案
Product Specific Protocols | |
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WB protocol for E-cadherin antibody 60335-1-Ig | Download protocol |
IHC protocol for E-cadherin antibody 60335-1-Ig | Download protocol |
IF protocol for E-cadherin antibody 60335-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
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