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Anti-Human E-selectin/CD62E Rabbit Recombinant Antibody, PBS Only

E-selectin/CD62E Uni-rAbTM Recombinant Antibody for FC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241767H6

Cat no : 98189-1-PBS

Print datasheet

Synonyms

CD62E, E-selectin, ELAM 1, ELAM, E selectin

验证数据展示

  • FC experiment of HUVEC using 98189-1-RR (same clone as 98189-1-PBS)

    FC experiment of HUVEC using 98189-1-RR (same clone as 98189-1-PBS)

    1x10^6 untreated or TNF alpha treated HUVEC cells were surface stained with 0.25 ug Anti-Human E-selectin/CD62E Rabbit Recombinant Antibody (98189-1-RR, Clone: 241767H6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98189-1-PBS in a different storage buffer formulation.

  • FC experiment of HUVEC using 98189-1-RR (same clone as 98189-1-PBS)

    FC experiment of HUVEC using 98189-1-RR (same clone as 98189-1-PBS)

    1x10^6 TNF alpha treated HUVEC cells were surface stained with 0.25 ug Anti-Human E-selectin/CD62E Rabbit Recombinant Antibody (98189-1-RR, Clone: 241767H6) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated HUVEC cells were surface stained with 0.25 ug Anti-Human E-selectin/CD62E Rabbit Recombinant Antibody (98189-1-RR, Clone: 241767H6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed. This data was developed using the same antibody clone with 98189-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01636-1

    Cytometric bead array standard curve of MP01636-1

    Cytometric bead array standard curve of MP01636-1, E-selectin / CD62E Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84887-2-PBS. Detection antibody: 98189-1-PBS. Standard: RP02341. Range: 0.313-40 ng/mL.

产品信息

98189-1-PBS targets E-selectin/CD62E as part of a matched antibody pair:

MP01636-1: 84887-2-PBS capture and 98189-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications FC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name selectin E
Synonyms CD62E, E-selectin, ELAM 1, ELAM, E selectin
Calculated Molecular Weight 610 aa, 67 kDa
GenBank Accession NumberBC142711
Gene Symbol CD62E
Gene ID (NCBI) 6401
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP16581
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

E-selectin, also known as CD62E or ELAM-1, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)