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验证数据展示

  • FC experiment of HUVEC using 98189-1-RR (same clone as 98189-1-PBS)

    FC experiment of HUVEC using 98189-1-RR (same clone as 98189-1-PBS)

    1x10^6 untreated or TNF alpha treated HUVEC cells were surface stained with 0.25 ug Anti-Human E-selectin/CD62E Rabbit Recombinant Antibody (98189-1-RR, Clone: 241767H6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98189-1-PBS in a different storage buffer formulation.

  • FC experiment of HUVEC using 98189-1-RR (same clone as 98189-1-PBS)

    FC experiment of HUVEC using 98189-1-RR (same clone as 98189-1-PBS)

    1x10^6 TNF alpha treated HUVEC cells were surface stained with 0.25 ug Anti-Human E-selectin/CD62E Rabbit Recombinant Antibody (98189-1-RR, Clone: 241767H6) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated HUVEC cells were surface stained with 0.25 ug Anti-Human E-selectin/CD62E Rabbit Recombinant Antibody (98189-1-RR, Clone: 241767H6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed. This data was developed using the same antibody clone with 98189-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01636-3

    Sandwich ELISA standard curve of MP01636-3

    Sandwich ELISA standard curve of MP01636-3, Human E-selectin / CD62E Recombinant Matched Antibody Pair - PBS only. 84887-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02341. 98189-1-PBS was HRP conjugated as the detection antibody. Range: 9.77-625 pg/mL

  • Sample test of MP01636-3

    Sample test of MP01636-3

    Serum of eight individual healthy human donors was measured. The human E-selectin / CD62E concentration of detected samples was determined to be 12.99 ng/mL with a range of 4.01 - 24.83 ng/mL.

  • Cytometric bead array standard curve of MP01636-1

    Cytometric bead array standard curve of MP01636-1

    Cytometric bead array standard curve of MP01636-1, E-selectin / CD62E Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84887-2-PBS. Detection antibody: 98189-1-PBS. Standard: RP02341. Range: 0.313-40 ng/mL.

E-selectin/CD62E Recombinant antibody, PBS Only (Detector)

E-selectin/CD62E Uni-rAbTM Recombinant Antibody for FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 98189-1-PBS

产品说明书

CloneNo. 241767H6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

CD62E, E-selectin, ELAM 1, ELAM, E selectin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

98189-1-PBS targets E-selectin/CD62E as part of a matched antibody pair:

MP01636-1: 84887-2-PBS capture and 98189-1-PBS detection (validated in Cytometric bead array)

MP01636-3: 84887-3-PBS capture and 98189-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 selectin E
别名 CD62E, E-selectin, ELAM 1, ELAM, E selectin
计算分子量 610 aa, 67 kDa
GenBank蛋白编号BC142711
基因名称 CD62E
Gene ID (NCBI) 6401
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP16581
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

E-selectin, also known as CD62E or ELAM-1, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)