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Anti-Human E-selectin/CD62E Rabbit Recombinant Antibody

E-selectin/CD62E Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241767H6

Cat no : 98189-1-RR

Print datasheet

Synonyms

CD62E, E-selectin, ELAM 1, ELAM, E selectin

验证数据展示

  • FC experiment of HUVEC using 98189-1-RR

    FC experiment of HUVEC using 98189-1-RR

    1x10^6 untreated or TNF alpha treated HUVEC cells were surface stained with 0.25 ug Anti-Human E-selectin/CD62E Rabbit Recombinant Antibody (98189-1-RR, Clone: 241767H6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • FC experiment of HUVEC using 98189-1-RR

    FC experiment of HUVEC using 98189-1-RR

    1x10^6 TNF alpha treated HUVEC cells were surface stained with 0.25 ug Anti-Human E-selectin/CD62E Rabbit Recombinant Antibody (98189-1-RR, Clone: 241767H6) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated HUVEC cells were surface stained with 0.25 ug Anti-Human E-selectin/CD62E Rabbit Recombinant Antibody (98189-1-RR, Clone: 241767H6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed.

经过测试的应用

Positive FC detected inTNF alpha treated HUVEC cells

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98189-1-RR targets E-selectin/CD62E in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name selectin E
Synonyms CD62E, E-selectin, ELAM 1, ELAM, E selectin
Calculated Molecular Weight 610 aa, 67 kDa
GenBank Accession NumberBC142711
Gene Symbol CD62E
Gene ID (NCBI) 6401
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP16581
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

E-selectin, also known as CD62E or ELAM-1, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)

实验方案

Product Specific Protocols
FC protocol for E-selectin/CD62E antibody 98189-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols