ECSIT Recombinant antibody, PBS Only

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

240223G2

Cat No : 83295-5-PBS

Synonyms

ECSIT, ECSIT homolog (Drosophila), Protein SITPEC, SITPEC

WB analysis using 83295-5-RR (same clone as 83295-5-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 83295-5-RR (ECSIT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83295-5-PBS in a different storage buffer formulation.
IF Staining of A431 using 83295-5-RR (same clone as 83295-5-PBS)
Immunofluorescent analysis of (4% PFA) fixed A431 cells using ECSIT antibody (83295-5-RR, Clone: 240223G2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83295-5-PBS in a different storage buffer formulation.
Affinity experiments of 83295-5-RR
Biolayer interferometry (BLl) kinetic assays of 83295-5-RR against Human ECSIT were performed. The affinity constant is 1.95 nM.

Size

The immunogen of 83295-5-PBS is ECSIT Fusion Protein expressed in E. coli.

Tested Applications	WB, IF, ELISA Application Description
Tested Reactivity	Human
Immunogen	ECSIT fusion protein Ag26898 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Full Name	ECSIT homolog (Drosophila)
Synonyms	ECSIT, ECSIT homolog (Drosophila), Protein SITPEC, SITPEC
Calculated Molecular Weight	49 kDa
GenBank Accession Number	BC008279
Gene Symbol	ECSIT
Gene ID (NCBI)	51295
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	Q9BQ95
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.