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ECSIT Recombinant antibody, PBS Only

ECSIT Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240223G2

Cat no : 83295-5-PBS

Print datasheet

Synonyms

SITPEC, Protein SITPEC, ECSIT homolog (Drosophila), 240223G2

验证数据展示

  • WB analysis using 83295-5-RR (same clone as 83295-5-PBS)

    WB analysis using 83295-5-RR (same clone as 83295-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83295-5-RR (ECSIT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83295-5-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 83295-5-RR (same clone as 83295-5-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 83295-5-RR (same clone as 83295-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83295-5-RR (ECSIT antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83295-5-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 83295-5-RR (same clone as 83295-5-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 83295-5-RR (same clone as 83295-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83295-5-RR (ECSIT antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83295-5-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 83295-5-RR (same clone as 83295-5-PBS)

    IF Staining of A431 using 83295-5-RR (same clone as 83295-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ECSIT antibody (83295-5-RR, Clone: 240223G2 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 83295-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83295-5-RR (same clone as 83295-5-PBS)

    FC experiment of HeLa using 83295-5-RR (same clone as 83295-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ECSIT Recombinant antibody (83295-5-RR, Clone:240223G2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83295-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83295-5-RR

    Affinity and Kinetic Characterization of 83295-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83295-5-RR against Human ECSIT were performed. The affinity constant is 1.95 nM.

产品信息

The immunogen of 83295-5-PBS is ECSIT Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen ECSIT fusion protein Ag26898 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ECSIT homolog (Drosophila)
Synonyms SITPEC, Protein SITPEC, ECSIT homolog (Drosophila), 240223G2
Calculated Molecular Weight 49 kDa
Observed Molecular Weight49 kDa
GenBank Accession NumberBC008279
Gene Symbol ECSIT
Gene ID (NCBI) 51295
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9BQ95
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ECSIT is a cytosolic adaptor protein associated with the toll-like receptor pathway. It has a distinct N-terminal mitochondrial targeting sequence, pentatricopeptide repeat motif, and a C-terminal pleckstrin homology domain. ECSIT regulates many biological processes like embryonic development, inflammation, cardiac function, and assembly of mitochondrial complex I. In macrophages, the activation of TLRs by inflammatory signals promotes the translocation of TRAF6 to mitochondria where it engages ECSIT to stimulate the production of mitochondrial reactive oxygen species (ROS).