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ECSIT Recombinant antibody

ECSIT Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240223G2

Cat No : 83295-5-RR

Print datasheet

Synonyms

ECSIT, ECSIT homolog (Drosophila), Protein SITPEC, SITPEC

验证数据展示

  • WB analysis using 83295-5-RR

    WB analysis using 83295-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83295-5-RR (ECSIT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 83295-5-RR

    IF Staining of A431 using 83295-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ECSIT antibody (83295-5-RR, Clone: 240223G2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Affinity experiments of 83295-5-RR

    Affinity experiments of 83295-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83295-5-RR against Human ECSIT were performed. The affinity constant is 1.95 nM.

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, A549 cells, HepG2 cells, HEK-293 cells
Positive IF detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83295-5-RR is ECSIT Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen ECSIT fusion protein Ag26898 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ECSIT homolog (Drosophila)
Synonyms ECSIT, ECSIT homolog (Drosophila), Protein SITPEC, SITPEC
Calculated Molecular Weight 49 kDa
Observed Molecular Weight49 kDa
GenBank Accession NumberBC008279
Gene Symbol ECSIT
Gene ID (NCBI) 51295
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9BQ95
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ECSIT is a cytosolic adaptor protein associated with the toll-like receptor pathway. It has a distinct N-terminal mitochondrial targeting sequence, pentatricopeptide repeat motif, and a C-terminal pleckstrin homology domain. ECSIT regulates many biological processes like embryonic development, inflammation, cardiac function, and assembly of mitochondrial complex I. In macrophages, the activation of TLRs by inflammatory signals promotes the translocation of TRAF6 to mitochondria where it engages ECSIT to stimulate the production of mitochondrial reactive oxygen species (ROS).

实验方案

Product Specific Protocols
WB protocol for ECSIT antibody 83295-5-RRDownload protocol
IF protocol for ECSIT antibody 83295-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols