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验证数据展示

  • IF Staining of HeLa using CL488-68065

    IF Staining of HeLa using CL488-68065

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 EEA1 antibody (CL488-68065, Clone: 1E11E4 ) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of HeLa using CL488-68065

    FC experiment of HeLa using CL488-68065

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human EEA1 (CL488-68065, Clone:1E11E4) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated EEA1 Monoclonal antibody

EEA1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68065

产品说明书

CloneNo. 1E11E4
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra) and More (1)

别名

ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, Early endosome antigen 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68065-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL488-68065 targets EEA1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
免疫原 EEA1 fusion protein Ag28814 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 early endosome antigen 1
别名 ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, Early endosome antigen 1
计算分子量 162 kDa
观测分子量 162 kDa
GenBank蛋白编号NM_003566
基因名称 EEA1
Gene ID (NCBI) 8411
RRIDAB_2919561
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ15075
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EEA1 antibody CL488-68065Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Autophagy

Macrophage autophagy protects against acute kidney injury by inhibiting renal inflammation through the degradation of TARM1

Authors - Xiao-Rong Huang
View Article