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验证数据展示

  • IF Staining of HepG2 using CL594-68065

    IF Staining of HepG2 using CL594-68065

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 EEA1 antibody (CL594-68065, Clone: 1E11E4 ) at dilution of 1:200, CL488-Phalloidin (green).

  • FC experiment of HeLa using CL594-68065

    FC experiment of HeLa using CL594-68065

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human EEA1 (CL594-68065, Clone:1E11E4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated EEA1 Monoclonal antibody

EEA1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-68065

产品说明书

CloneNo. 1E11E4
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra) and More (1)

别名

ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, Early endosome antigen 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68065-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL594-68065 targets EEA1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
免疫原 EEA1 fusion protein Ag28814 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 early endosome antigen 1
别名 ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, Early endosome antigen 1
计算分子量 162 kDa
观测分子量 162 kDa
GenBank蛋白编号NM_003566
基因名称 EEA1
Gene ID (NCBI) 8411
RRIDAB_2920189
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ15075
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.

实验方案

Product Specific Protocols
IF protocol for CL594 EEA1 antibody CL594-68065Download protocol
FC protocol for CL594 EEA1 antibody CL594-68065Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Mol Pharm

Glatiramer Acetate Complexes CpG Oligodeoxynucleotides into Nanoparticles and Boosts Their TLR9-Driven Immunity

Authors - Huan Gong
View Article