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验证数据展示

  • WB analysis using 67756-1-Ig

    WB analysis using 67756-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67756-1-Ig (EED antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of mouse testis using 67756-1-Ig

    IHC staining of mouse testis using 67756-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67756-1-Ig (EED antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 67756-1-Ig

    IHC staining of mouse testis using 67756-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67756-1-Ig (EED antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 67756-1-Ig

    IF Staining of mouse testis using 67756-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using EED antibody (67756-1-Ig, Clone: 1D11D4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse testis using 67756-1-Ig

    IF Staining of mouse testis using 67756-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using EED antibody (67756-1-Ig, Clone: 1D11D4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

EED Monoclonal antibody

EED Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67756-1-Ig

产品说明书

CloneNo. 1D11D4

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

EED, embryonic ectoderm development, HEED, Polycomb protein EED, WAIT 1, WAIT1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, THP-1 cells, HEK--293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67756-1-Ig targets EED in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 EED fusion protein Ag10369 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 embryonic ectoderm development
别名 EED, embryonic ectoderm development, HEED, Polycomb protein EED, WAIT 1, WAIT1
计算分子量 466 aa, 53 kDa
观测分子量50/53/46 kDa.
GenBank蛋白编号BC068995
基因名称 EED
Gene ID (NCBI) 8726
RRIDAB_2918524
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75530
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED has three isoforms, with the molecular weight of isoforms 1/2/3: 50/53/46 kDa.

实验方案

Product Specific Protocols
WB protocol for EED antibody 67756-1-IgDownload protocol
IHC protocol for EED antibody 67756-1-IgDownload protocol
IF protocol for EED antibody 67756-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols